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| Effect of Carbon, Nitrogen Sources on Lipid Accumulation and Fatty Acid Composition of Monoraphidium sp. FXY-10 under Heterotrophic Cultivation |
| HUANG Li, HE Ci-an, ZHAO Peng, YU Xu-ya |
| College of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China |
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Abstract Effect of carbon and nitrogen sources on heterotrophic cultivation of microalgae Monoraphidium sp. FXY-10 is studied. The biomass, oil accumulation and fatty acid composition of microalgae cell are analysed comparatively by selecting various types and setting different concentrations of carbon and nitrogen sources with BG-11 as basic medium. The results show that microalgae lipid accumulating with glucose as carbon source and sodium nitrate as nitrogen source is an ideal candidate for biodiesel production. The lipid production is not significantly impacted (P>0.05) by different sodium nitrate concentration which was 1.00, 3.00 and 5.00 g/L, respectively. Moreover, when glucose concentration is 10.00 g/L using sodium nitrate as nitrogen source, lipid production can be obtained the highest experiment value 0.84 g/L. And the fatty acid of microalgae lipid is mainly composed of short chain saturated and monounsaturated fatty acids, such as C16:0 and C18:1. So they result a lower degree of unsaturation (DU) value 61.98.
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Received: 15 June 2012
Published: 25 February 2013
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