Optimization of Carbon and Nitrogen Source of Basic Liquid Culture of <em>Gymnopus</em> sp. Producing Anti-fungal Activity Substance and Its Effect on Green and Blue Mold of Citrus

doi:10.13523/j.cb.20160208

China Biotechnology

2016, Vol. 36

Issue (2): 51-61 DOI: 10.13523/j.cb.20160208

Optimization of Carbon and Nitrogen Source of Basic Liquid Culture of Gymnopus sp. Producing Anti-fungal Activity Substance and Its Effect on Green and Blue Mold of Citrus

WU Sheng, HUO Guang-hua, HAN Qi-can, LONG Hao-zhi

Nanchang Key Laboratory of Bioresources Coservation and Utilization, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China

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Abstract

Jiangxi is one of the main producing area of citrus fruits. Green and blue mold are the cause of major diseases of post-harvest decay of citrus fruits. Chemical fungicides have generated pathogen resistance and residual. A macrofungal strain Gymnopus sp. 0612-9 with activity of antagonistic the pathogens was found. In order to investigate the metabolic culture of producing active substance and its action against Green and blue mold, the anti-fungal activity was determined by the method of mycelium growth rate and microscopic observation. The medium composition and culture conditions were optimized by the single factor test, Plackett-Burman, steepest ascent test and the response surface Box-Behnken design. The results showed the liquid medium composition and culture conditions are potato 279.94g/L, glucose 30g/L, bran 8.87g/L, MgSO₄·7H₂O 0.47g/L, KH₂PO₄ 2g/L; 10 days of fermentation period, inoculation volume 5ml/100ml, shaking speed 180r/min, culture temperature 26℃, liquid medium volume 100ml/250ml with the anti-pathogen activity of the fermentation broth increased from 42.26% to 55.77%. The EC₅₀ value against Penicillium italicum and Penicillium digitatum are 69.25μg/ml and 56.70μg/ml, respectively. The minimum fungicidal concentration(MFC) is 250μg/ml, which was lower than the positive control of imazalil of 500μg/ml and it has some toxic effects on the mycelium of the pathogens. The strain of Gymnopus sp. has potential value to develop into antiseptic agent of citrus fruits.

Key words： Active substance Strain of Gymnopus sp.0612-9 Carbon and nitrogen source optimization Action of against green and blue mold

Received: 20 November 2015 Published: 14 December 2015

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WU Sheng, HUO Guang-hua, HAN Qi-can, LONG Hao-zhi. Optimization of Carbon and Nitrogen Source of Basic Liquid Culture of Gymnopus sp. Producing Anti-fungal Activity Substance and Its Effect on Green and Blue Mold of Citrus. China Biotechnology, 2016, 36(2): 51-61.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160208 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I2/51

[1] 马俊莲,张子德. 我国果蔬贮藏保鲜业现状与发展对策. 保鲜与加工,2001,01:28-30. Ma J L,Zhang Z D.Status and developing strategies for fruit and vegetables storage in China. Storage & Process,2001,01:28-30.
[2] 盛爱武,乔爱民,高飞,等. 富贵竹切口保鲜及贮运研究. 中国农学通报,2005,09:329-331. Sheng A W,Qiao A M,Gao F,et al.Studies on cutting surface preservative and storage and transport of Dracaena sanderiana. Chinese Agricultural Science Bulletin,2005,09:329-331.
[3] 田世平,范青. 控制果蔬采后病害的生物学技术. 植物学通报,2000,03:211-217. Tian S P,Fan Q. Biological technologies for the control of post-harvest diseases of fruits and vegetables.Chinese Bulletin of Botany,2000,03:211-217.
[4] Zhang H Y,Zheng X D,Fu C X,et al. Post-harvest biological control of gray mold rot of pare with Cryptococcus laurentii. Biology and Technology,2005,35:79-86.
[5] 习柳,田世平. 酵母拮抗菌与碳酸氢钠配合对番茄果实采后病害的防治效果研究. 中国农业科学,2005,05:950-955. Xi L,Tian S P. Control of postharvest diseases of tomato fruit by combining antagonistic yeast with sodium bicarbonate.Scientia Agricultura Sinica,2005,05:950-955.
[6] 康卓. 中国生物源农药产业化进展. 农药,2001,03:4-8. Kang Z.Current industrial advances of bio-pesticide in China. Agricultural Chemical,2001,03:4-8.
[7] 卯晓岚. 中国食用菌物种资源回顾与展望. 中国食用菌,2000,19(增刊):9-13. Mou X L.Review and prospect of the species resources of edible fungi in China. Edible Fungi of China, 2000,19(extra edition):9-13.
[8] Mier N,Canete S,Klaebe A,et al. Insecticidal properties of mushroom and toadstool carpophores. Phytochemistry,1996,41(5):1293-1299.
[9] Stadler M,Sterner O. Production of bioactive secondary metabolites in the fruit bodies of macro fungi as a response to injury. Phytochemistry,1998,49(4):1013-1019.
[10] 韩启灿,霍光华,罗桂祥,等. 野生冬菇产病原拮抗物的工艺参数确定.中国预防兽医学报,2014,03:208-213. Han Q C,Huang G H,Luo G X,et al. The fermentation process of anti-pathogenic bacteria substances produced by a wild Flammulina ssp. Chinese Journal of Preventive Veterinary Medicine,2014,03:208-213.
[11] 李跃进,何晓兰,李泰辉. 中国食用菌已知科属的系统排列. 食用菌学报,2010,03:78-86. Li Y J,He X L,Li T H. Systematic arrangement of the families and genera of edible fungi reported in China. Acta Edulis Fungi,2010,03:78-86.
[12] Halling R E. The genus Collybia(Agaricales) in the Northeastern United States and adjacent Canada. Mycologia Memoir,Cramer Publ,1983,8:148.
[13] Antonín,V,Noordeloos M E. A monograph of Marasmius,Collybia and related genera in Europe. Part 2:Collybia,Gymnopus,Rhodocollybia,Crinipellis,Chaetocalathus,and additions to Marasmiellus. Libri Botanici,1997,17:256.
[14] Antonin V,Finy P,Tomsovsky M. Taxonomy of the Gymnopus inusitatus group and the new G. inusitatus var. cystidiatus from Hungary. Mycotaxon,2012,119(1):291-299.
[15] Mata J L,Ovrebo C L. New reports and illustrations of Gymnopus for Costa Rica and Panama. Fungal Diversity,2009,38:125.
[16] Miyamoto T,Igarashi T. Gymnopus piceipes sp. nov. from Japan. Mycoscience,2001,42(2):177-180.
[17] Banares A,Antonín V,Moreno G. Gymnopus beltraniae,a new species of section Vestipedes(Agaricales) from the Canary Islands(Spain). Persoonia-Molecular Phylogeny and Evolution of Fungi,2007,19(2):255-259.
[18] 李跃进,李泰辉,图力古尔.中国裸伞属已知种的分布及1个大陆新记录种.菌物研究,2012,01:8-12. Li Y J,Li T H,Tu L G L.Distribution of known species of Gymnopilus from China and a new record to the Chinese mainland.Journal of Fungal Research,2012,01:8-12.
[19] 易磊,霍光华,韩启灿,等. 木荷皂甙对稻瘟病菌细胞形态及生理生化指标的影响. 植物保护学报,2013,05:450-456. Yi L,Huo G H,Han Q C.Effects of saponins from Schima superba on cell morphology,physiological and biochemical indices of Magnaporthe oryzae. Acta Phytophylacica Sinica,2013,05:450-456.
[20] 吴振宇,王燕,艾启俊.鹿蹄草素对桃褐腐病菌的抑制作用及其抑菌机理.中国农业科学,2009,08:2784-2792. Wu Z Y,Wang Y,Ai Q J. Inhibitory effect and antimicrobial mechanism of pyrolin on Monilinia fructicola in peach.Scientia Agricultural Sinica,2009,08:2784-2792.
[21] Yang S,Peng L,Cheng Y,et al. Control of citrus green and blue molds by Chinese propolis. Food Science and Biotechnology,2010,19(5):1303-1308.
[22] Sholberg P L,Shimizu B N. Use of natural plant product,hinokitiol,to extend shelf-life of peaches. Can Inst Food Sci Technol,1991(2):273-276.
[23] 席钦芳,瞿传清,郑永华. 植物杀菌素抑制柑桔青绿霉菌的研究(简报). 浙江农业大学学报,1994,02:169-170. Xi Q F,Zhai C Q,Zheng Y H.Studies on the inhibitory effects of phytotoxins on Penicillium italicum Wehmer and P. Digitatum Sacc. Journal of Zhejiang Agricultural University,1994,02:169-170.
[24] Wilson C L,Franklin J D,Otto B E. Fruit volatiles inhibitory to Monitinia fructicola and Botrytis cinerea. Plant Dis,1987,71:316.
[25] 曾志恒,郑怡,刘艳如,等. 凤尾菇深层发酵液抗细菌抗真菌活性的研究. 福建师范大学学报(自然科学版),2010,03:72-77. Zeng Z H,Zheng Y,Liu Y R,et al.Study on antibacterial and antifungal activities of liquid from submerged fermentation of Pleurotus sajor-caju. Natural Science Journal of Fujian Normal University,2010,03:72-77.

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