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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (11): 46-51    DOI: 10.13523/j.cb.20151107
    
Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp. FXY-10 Under Mixotrophic and Heterotrophic Cultivations
CHE Rao-qiong, HUANG Li, WANG Lin, ZHAO Peng, LI Tao, YU Xu-ya
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
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Abstract  

The effects of initial glucose concentrations on cell growth of Monoraphidium sp.FXY-10 under mixotrophic and heterotrophic cultivations were investigated by Andrew equation.A kinetic model was developed to describe the dynamics of glucose consumption for FXY-10 growth. Meanwhile,the influence of different glucose concentrations on the production of lipid by FXY-10 was studied. And the kinetic models of FXY-10 lipid synthesis in the different trophic types with the most appropriate glucose concentration were also constructed. The results showed thatwhen the concentration of glucose was 10g/L, the maximum total lipid content and biomass concentration were achieved under mixotrophic and heterotrophic conditions. The semi-saturation constants of Monoraphidium sp. FXY-10 to glucose in the mixotrophic(KSJ) and heterotrophic (KSY)conditions were 1.89g/L and 17.82g/L, respectively. It implied that the glucose absorption capacity of FXY-10 in the mixotrophic condition was relatively higher than that in the heterotrophic.



Key wordsMonoraphidium sp. FXY-10      Heterotrophic      Mixotrophic      Glucose      Lipid content     
Received: 29 June 2015      Published: 25 November 2015
ZTFLH:  Q493.4  
Cite this article:

CHE Rao-qiong, HUANG Li, WANG Lin, ZHAO Peng, LI Tao, YU Xu-ya. Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp. FXY-10 Under Mixotrophic and Heterotrophic Cultivations. China Biotechnology, 2015, 35(11): 46-51.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20151107     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I11/46

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