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Screening Oleaginous Microalgae and Evaluation of the Oil-producing Charateristic |
LI Tao1, LI Ai-fen1, SANG Min1, WU Hong2, YIN Shun-ji2, ZHANG Cheng-wu1 |
1. Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering,Ministry of Education, Guangzhou 510632,China;
2. State Key Laboratory of Coal-Based Low-Carbon Energy, Xinao Scientific & Technological Developmental Co. Ltd., Langfang 065001,China |
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Abstract Twenty strains of freshwater and marine microalgae collected in our laboratory which belong to Chlorophytes, Eustigmatophytes and Bacillariophytes were screened for their potential of oil production. All microalgae were cultured in batch, with bubble column photobioreactor (light path, 3 cm). These microalgae were evaluated by measuring dry weight, total lipid content, total lipid yield, total lipid volumetric productivity. The results showed that the dry weight and total lipid contents of twenty microalgae were in the range of 1.81~7.88g/L and 16.0%~55.9% dw, respectively, and nine of them were referred as oleaginous microalgae, including Scenedesmus sp.(6.34g/L, 55.9% dw), Chlorococcum tatrense(5.93g/L, 46.9% dw), Nannochloropsis oculata(7.88g/L, 35.0% dw), Chlorococcum oleofaciens(5.58g/L, 45.9% dw), Pseudochlorococcum polymorphum (6.10g/L, 40.0% dw), Chlamydomonas augustae(5.78g/L, 40.5% dw), Chlorella ellipsoidea(5.56g/L, 40.7% dw), Chlorococcum ellipsoideum(5.41g/L, 38.0% dw), Chlorococcum nivale(5.55g/L, 36.3% dw). It was concluded that the most potential for industrial application was Scenedesmus sp, which lipid yield and lipid volumetric productivity was 3.5g/L and 218.7mg/L·d, respectively.
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Received: 08 October 2010
Published: 26 April 2011
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[1] Brennan L, Owende P. Biofuels from microalgae-A review of technologies for production, processing, and extractions of biofuels and co-products. Renew Sustain Energy Rev, 2010, 14: 557-577.
[2] Hu Q,Sommerfeld M,Jarvis E, et al. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J, 2008, 54: 621-639.
[3] Ma F, Hanna M A. Biodiesel production: a review. Bioresource Technol, 1999, 70: 1-15.
[4] 周良虹,黄亚晶.国外生物柴油产业与应用状况.可再生能源,2005,122(4):62-67. Zhou L H, Huang Y J. Renwable Energy, 2005, 122(4): 62-67.
[5] Williams P, Laurens L. Microalgae as biodiesel and biomass feedstocks: review and analysis of the biochemistry and economics. Energy Envion Sci, 2010, 3:554-590.
[6] Chisti Y. Biodiesel from microalgae. Biotechnol Adv, 2007, 25: 294-306.
[7] Singh J, Gu S. Commercialization potential of microalgae for biofuels production. Renew Sustain Energy Rev, 2010, 14(9):2596-2610.
[8] Sheehan J, Dunahay T,Benemann J, et al. A look back at the US depart ment of energy’s aquatic species program: biodiesel from algae. U.S. Report NREL, 1998, 1-64.
[9] 梅洪,张成武,殷大聪,等.利用微藻生产可再生能源研究概况.武汉植物学研究,2008,26(6):650-660. Mei H, Zhang C W,Yin D C, et al. Journal of Wuhan Botanical Research, 2008, 26(6):650-660.
[10] Mutanda T, Ramesh D,Karthikeyan S, et al. Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production. Bioresource Technol, 2011, 102(1): 57-70.
[11] Griffiths M J, Harrison S T L. Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol, 2009, 21: 493-507.
[12] Rodolfi L, Zittelliv C G,Bassi N, et al. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng, 2009, 102(1): 100-112.
[13] Feng Y, Li C,Zhang D W, et al. Lipid production of Chlorella vulgaris cultured in artificial waste water medium. Bioresource Technol, 2011, 102(1): 101-105.
[14] 蒋汉明,高坤山.氮源及其浓度对三角褐指藻生长和脂肪酸组成的影响.水生生物学报 2004,28(5):545-551. Jiang H M, Gao K S. Acta Hydrobiologica Sinica, 2004, 28(5):545-551.
[15] 曹春晖,孙世春,麦康森,等.光照强度对四株海洋绿藻总脂含量和脂肪酸组成的影响.生态学报,2010,30(9):2347-2353. Cao C H, Sun S C,Mai K S, et al. Acta Ecologica Sinica, 2010, 30(9):2347-2353.
[16] 俞建江,李荷芳,周汉秋,等. 10种海洋微藻总脂、中性脂和极性脂的脂肪酸组成.水生生物学报,1999,23(5):481-488. Yu J J, Li H F,Zhou H Q, et al. Acta Hydrobiologica Sinica, 1999, 23(5):481-488.
[17] 梁英,麦康森,孙世春,等.角毛藻属7株总脂含量及脂肪酸组成的比较研究.海洋湖沼通报,2000,3:29-33. Liang Y, Mai K S,Sun S C, et al. Transactions of Oceanology and Limnology, 2000,3:29-33.
[18] 王金娜,严小军,周成旭,等.产油微藻的筛选及中性脂动态积累过程的检测.生物物理学报,2010,26(6):472-480. Wang J N,Yan X J,Zhu C X, et al. Acta Biophysica Sinica, 2010, 26(6):472-480.
[19] 王明清,迟晓元,秦松,等.海洋微藻总脂含量和脂肪酸组成的测定.中国油脂,2008,33(11):67-70. Wang M Q, Chi X Y,Qin S, et al. China Oils and Fats, 2008, 33(11):67-70.
[20] Richmond A. Handbook of Microalgal Culture:Biotechnology and Applied Phycology. Blackwell, 2004.
[21] Zhang C W, Zmora O,Kopel R, et al. An industrial-size flat plate glass reactor for mass production of Nannochloropsis sp.(Eustigmatophyceae). Aquaculture, 2001, 195: 35-49.
[22] Jensen A. Chlorophylls and Carotenoids. Handbook of Phycological Methods. Cambridge, 1978. 60-70.
[23] Khozin-Goldberg I, Shrestha P, Cohen Z. Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa. Biochimica et Biophysica Acta, 2005, 1738: 63-71.
[24] 夏金兰,万民熙,王润民,等.微藻生物柴油的现状与进展.中国生物工程杂志,2009,29(7):118-126. Xia J L, Wan M X,Wang R M, et al. China Biotechnology, 2009, 29 (7) : 118-126.
[25] 王立柱,温皓程,邹渝,等.产油微藻的分离、筛选及自养培养氮源、碳源的优化.微生物学通报,2010,37(3):336-341. Wang L Z, Wen H C,Zou Y, et al. Microbiology China, 2010, 37(3):336-341.
[26] Sobczuka T M, Chisti Y. Potential fuel oils from the microalga Choricystis minor. J Chem Technol Biotechnol, 2010, 85:100-108.
[27] De la Pena M R. Cell growth and nutritive value of the tropical Benthic diatom, Amphora sp., at varying levels of nutrients and light intensity, and different culture locations. J Appl Phycol, 2007, 19: 647-655.
[28] Li Y,Horsman M,Wang B, et al. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol, 2008, 81:629-636.
[29] 姜进举,苗凤萍,冯大伟,等. 微藻生物柴油技术的研究现状及展望. 中国生物工程杂志,2010, 30(2):134-140. Jiang J J, Miao F P, Feng D W, et al. China Biotechnology, 2010, 30(2):134-140.
[30] National Algal Biofuels Technology Roadmap. 2008. U.S. Department of Energy.
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