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| The Growth and Lipids Accumulation Pattern of Oleaginous Green Microalga Scenedesmus acuminatus Large Volume Cultured in Flat Panel Photobioreactor |
| LEI Xue-qing, LU Zhe, GAO Bao-yan, ZHANG Wen-yuan, LI Ai-fen, ZHANG Cheng-wu |
| Research Center of Hydrobiology, Depart of Ecology, Jinan University, Guangzhou 510632, China |
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Abstract Scenedesmus acuminatus was a new isolated freshwater green microalga cultured in modified BG-11 medium. In order to improve the rapid accumulation of the lipids, the initial NaNO3 concentration reduced to one third and one fifth of the original NaNO3 concentration in the BG-11 medium, 6.0mmol/L and 3.6mmol/L, respectively. It was large volume cultured in a new-designed internally installed tiepiece flat panel photobioreactor. To analyze the growth and oil accumulation pattern of S.acuminatus mass cultures, the biomass, total lipids content, lipid compositions, and fatty acids profiles in different phase were investigate. When the initial NaNO3 concentration was 6.0mmol/L, the biomass(6.27g/L)was higher than the biomass(5.30g/L) of 3.6mmol/L treatment. While, the highest lipids content of 56.6% of dry weight was occurred at 3.6mmol/L treatment. The total lipids content was fractionated by solid phase extraction (SPE) into three broad classes: neutral lipid (NL), glycolipid (GL) and phospholipid (PL). The content of neutral lipid increased along with the culture time, and it reached to 90.9% and 92.0% of the total lipids, 47.5% and 51.4% of dry weight when the initial NaNO3 concentration was 6.0mmol/L and 3.6 mmol/L, respectively. The major fatty acids of S.acuminatus were C16:0, C16:1, C18:0, C18:1, C18:2,and C18:3,which together accounted for 89.9%~96.2% of the total fatty acids content and 12.5%~50.7% of dry weight. The volumetric productivity of total lipids, neutral lipids and total fatty acids of S.acuminatus were 0.18g/L·d, 0.16 g/L·d and 0.15 g/L·d when the initial NaNO3 concentration was 6.0mmol/L and 0.16g/L·d, 0.15g/L·d and 0.15g/L·d when the initial NaNO3 concentration was 3.6 mmol/L, respectively. The results showed that S.acuminatus was a hyper-oil producing strain that is easy to large-scale cultivation and its profiles of fatty acids is suitable for biodiesel production.
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Received: 05 September 2014
Published: 25 November 2014
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