Study on On-line Capacitance Measurement to Evaluate the Viable Biomass During the Fermentation of <em>Pichia</em>

doi:10.13523/j.cb.20140313

China Biotechnology

2014, Vol. 34

Issue (3): 91-95 DOI: 10.13523/j.cb.20140313

Study on On-line Capacitance Measurement to Evaluate the Viable Biomass During the Fermentation of Pichia

LI Lan¹, WANG Ze-Jian¹, JIN Yong², SUN Wen-hua², ZHUANG Ying-ping¹, ZHANG Si-liang¹

1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai China;
2. Zhejiang Puchuang Instrument Co., LTD, Shanghai, China

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Abstract The amount of viable biomass is an important physiological parameter, which is correlated with the cell growth, metabolism and productivity in fermentations. Biomass Monitor is becoming an established tool for monitoring the capacitance to evaluate the concentration of viable biomass in the cell suspension. In this paper, the principle of the Biomass Monitor and the application in the Pichi fermentation is described. The results show that Biomass Monitor can directly obtain reliable amount of viable biomass in real-time during the fermentation process, and effectively avoid the interference of taking samples. This technology meets Process Analytical Technology (PAT) requirements, and it can be a useful tool combined with other data for the development, optimization, monitoring and control in fermentation processes.

Key words： Biomass monitor Viable biomass On-line capacitance measurement Pichia PAT

Received: 21 October 2013 Published: 25 March 2014

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	SUN Wen-hua
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	ZHANG Si-liang

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LI Lan, WANG Ze-Jian, JIN Yong, SUN Wen-hua, ZHUANG Ying-ping, ZHANG Si-liang. Study on On-line Capacitance Measurement to Evaluate the Viable Biomass During the Fermentation of Pichia. China Biotechnology, 2014, 34(3): 91-95.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140313 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I3/91

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