Optimization of (R)-Mandelic acid Fermentation Medium by Using Response Surface Methodology

doi:Q819

China Biotechnology

2010, Vol. 30

Issue (08): 112-117 DOI: Q819

Optimization of (R)-Mandelic acid Fermentation Medium by Using Response Surface Methodology

RUAN Wen-bing,CHEN Bi-qin,CHEN Su-hua,CHEN Bing-mei,XU Xiao-ping

Collage of Chemistry and Chemical Engineeing，Fuzhou University，Fuzhou 350108，China

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Abstract

Plackett-burman and response surface methodology were applied to optimize the Bacillus sp.HB20 fermentation medium components for synthesizing（R）-mandelic acid. The important factors influencing the yield of（R）-mandelic acid, which identified by initial screening method of Plackett–Burman，were Peptone, Beef extract and Maltose. The path of steepest ascent was undertaken to approach the optimal region of the three significant factors. Box–Behnken design and response surface methodology were adopted to further investigate the mutual interaction between the variables and identify optimal values that bring maximum（R）-mandelic acid yeild. Experimental results showed that Peptone, Beef extract and Maltose concentration all had significant influence on the specific（R）-mandelic acid yeild potential (Ps). The optimal conditions for the maximal Ps were: Peptone 11.507 g/L, Beef extract 6.708 g/L and Maltose 10.907 g/L, the maximum predicted value of（R）-mandelic acid yeild was 66.87%. After the checking experiment , the maximum predicted value was consistent with mean value of verification test and the （R）-mandelic acid yeild increased 25.87% at optimized condition.

Key words： (R)-mandelic acid Culture medium optimization Response surface methodology

Received: 05 January 2010 Published: 25 August 2010

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	RUAN Wen-Bing
	CHEN Bi-Qin
	CHEN Su-Hua
	CHEN Bing-Mei
	HU Xiao-Beng

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RUAN Wen-Bing, CHEN Bi-Qin, CHEN Su-Hua, CHEN Bing-Mei, HU Xiao-Beng. Optimization of (R)-Mandelic acid Fermentation Medium by Using Response Surface Methodology. China Biotechnology, 2010, 30(08): 112-117.

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https://manu60.magtech.com.cn/biotech/Q819 OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I08/112

［1］何晓强.扁桃酸的工业合成法. 贵州化工,2007,32(1):1112. He X Q. Guizhou Chemical Industry, 2007,32(1):1112.
［2］臧健,王志军,吴怡祖.扁桃酸的光学拆分. 精细化工中间体,2005,35(1):5052. Zang J, Wang Z J, Wu Y Z. Fine Chemical Intermediates, 2005,35(1):5052.
［3］ Ariens E. Nonchiral, homochiral and composite chiral drugs. TIBS, 1993, 14:6876.
［4］ Lilly M. Advances in biotransformations processes. Chem Eng Sci，1994, 49: 151159.
［5］杨元营,李忠琴,许小平.基于SASV8.1的黄嘌呤氧化酶产酶培养基的优化.计算机与应用化学, 2008,25(5):611614. Yang Y Y, LI Z Q, Xu X P. Computers and Applied Chemistry, 2008, 25(5):611614.
［6］熊智强,徐平,涂国全.利用响应面法优化红谷霉素发酵培养基.微生物学通报,2006,33(4):59. Xiong Z Q, Xu P, Tu G Q. Microbiology, 2006, 33(4):59.
［7］陈必钦,杨凯,陈倍,等. (R)肾上腺素高产菌株发酵培养基的优化.计算机与应用化学,2009， 26(7):935939. Chen B Q, Yang K, Chen B. Computers and Applied Chemistry, 2009, 26(7):935939.
［8］ Muralidhar R V, Chirumamila R R， Marchant R,et al. A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources. Biochcem Eng, 2001, 9(1):1723.
［9］张巧艳,钱俊青.响应面法优化黄杆菌突变株产脂肪酶摇瓶发酵条件.浙江工业大学学报, 2009, 37(2):156160. Zhang Q Y,Qian J Q. Journal of Zhejiang University of Technology, 2009, 37(2):156160.
［10］ Li C, Bai J H, Cai Z L. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. Journal of Biotechnology, 2002, 93:2734.
［11］ Ambat P, Ayyanna C. Optimizing medium constituents and fermentation conditions for citic production from palmyra jaggery using response surface methods. World Journal of Microbiology & Biotechnology, 2001, 17: 331335.
［12］ Ratnam B V V, Narasimha Rao M, Dmodar Rao M, et al. Optimization of fermentation conditions for the production of ethanol from sago starch using response surface methodology. World Journal of Microbiology & Biotechnology ,2003, 19(15): 523526.
［13］ Trupkin S, Levin S, Forchiassin F. Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology. J Ind Microbiol Biotechnol, 2003, 30(12): 682690.
［14］曹小红，蔡萍，李凡，等. 利用响应面法优化Bacillus natto TK1 产脂肽发酵培养基. 中国生物工程杂志，2007，27(4): 5965. Cao X H, Cai P, Li F. China Biotechnology, 2007, 27(4): 5965.
［15］代文亮,程龙,陶文沂.响应面法在紫杉醇产生菌发酵前体优化中的应用.中国生物工程杂志,2007, 27(11): 6672. Dai W L, Cheng L, Tao W Y. China Biotechnology, 2007, 11: 6672.
［16］吕国英，张作法，潘慧娟，等. 响应面分析法优化香菇多糖发酵培养基. 菌物学报. 2010, 29(1): 106112. Lv G Y, Zhang Z F, Pan H J. Mycosystema, 2010, 29(1): 106112.
［17］廖春丽，王福梅，陈兰英，等. 响应面方法在优化β胡萝卜素培养基的应用. 中国酿造. 2009(4):115117. Liao C L, Wang F M, Chen L Y. China Brewing, 2009(4): 115117.
［18］江宏文，张毅，李娜，等. 酵母菌株产麦角固醇发酵条件优化. 现代食品科技. 2009，25 (7): 800803. Jiang H W, Zhang Y, Li N, Modern Food Science and Technology, 2009, 25 (7): 800803.
［19］ Senthilkumar S R, Dem Psey M, Krishnan C, et al. Optimization of biobleaching of paper pulp in an expanded bed bioreactor with immobilized alkali stable xylanase by using response surface methodology. Bioresour Technol, 2008, 99 (16) :7781  7787.
［20］ Deepak V, Kalishwaralal K, Ramkumarpandian S, et al. Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour Technol, 2008, 99 ( 17 ) :8170  8174.
［21］王允祥,吕凤霞,陆兆新.杯伞发酵培养基的响应曲面法优化.南京农业大学学报,2004,27(3):8994. Wang Y X, Lv F X, Lu Z X. Journal of Nanjing Agricultural University, 2004, 27(3): 8994.

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