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| Fermentation Conditions Optimization of Deep Sea Gene Engineering Strain LIP001 Producing Cold-adaptive Lipase |
| CHAN Zhu-hua1, LIU Yang1, SU Yu-bin1, SHAN Da-peng1, WANG Shui-qi2, ZENG Run-ying1 |
1. Third Institute of Oceanography, SOA, Xiamen 361005,China;
2. Zhangzhou Institute of Technology, Zhangzhou 363000,China |
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Abstract One clone (LIP001) producing cold-active lipase was selected from metagenomic library of deep sea sediment for the optimization study of fermentation conditions. The effects of main factors on the growth and lipase-producing of LIP001 were determined by single-factor test. The results showed that the optimal condition were 30℃, pH7.0, and inoculum size 5%, liquid volume 50ml in 250ml flask, respectively. The fermentation conditions for the lipase-producing of LIP001 were further optimized by orthogonal experiment on the basis of single factor test. The major factors and their concentrations were as follows: olive oil (1%), yeast extract(0.5%), peptone (1%), (NH4)2SO4 (0.5%), phosphate (0.5%), MgSO4 (0.2%), and chloromycetin(12.5μg/ml), respectively. Under the optimized condition, the lipase activity was 1980U/ml which was increased by 54.7%. For 5L fermentor research, the lipase activity was 2420U/ml.
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Received: 28 October 2010
Published: 26 April 2011
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[1] 吴文惠,许剑锋,刘克海,等.海洋生物资源的新内涵及其研究与利用,科技创新导报,2009,18(4):98-99. Wu W H, Xu J F, Liu K H.Science and Technology Innovation Herald, 2009,18(4):98-99.
[2] 李越中,陈琦.海洋微生物资源多样性.生物工程进展,1998,18(4):34-40. Li Y Z, Chen Q. Progress in Biotechnology, 1998, 18(4):34-40.
[3] Federica S, Linda C. Biodiversity and potentials of marine-derived microorganisms. Journal of Biotechnology, 1999, 70 : 65-69.
[4] Hyun-Sook L, Kae-Kyoung K. Approaches for novel enzyme discovery from marine environments. Current Opinion in Biotechnology, 2010, 21:1-5.
[5] Shintrem S, Ghadge R S. Lipase catalyzed synthesis of benzyl esters of fatty acids.Biochemical Engeineering, 2002, 12(2): 131-141.
[6] Yang X X, Lin X Z. Identification of five strains of antarctic bacteria producing low-temperature lipase. Acta Oceanologica Sinica,2004, 23:717-723.
[7] Loreto P P, Fernando R. Cloning and fusion expression of a cold-active lipase from marine Antarctic origin. Enzyme and Microbial Technology,2008,42:371-377.
[8] Jeanette W P, Teo A, Lian-Hui Z,et al. Cloning and characterization of a novel lipase from Vibrio harveyi strain AP6. Gene, 2003,312 :181-188.
[9] 林学政.南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究.海洋学报,2005,27(3):154-158. Lin X Z.Acta Oceanologica Sinica,2005,27(3):154-158.
[10] 孙谧,邵铁娟,郑家声,等.Bohaisea29145海洋低温碱性脂肪酶研究.微生物学报,2004,44(6):789-793. Sun M, Shao T J, Zheng J S, et al.Acta Microbiologica Sinica,2004,44(6):789-793.
[11] 宋庆训,林金萍,魏东芝,等.脂肪酶产生菌Candida rugosa产酶条件研究.生物工程学报, 2001,17(1):101-104. Song Q X, Lin J P, Wei D Z, et al.Chinese Journal of Biotechnology,2001,17(1):101-104.
[12] 郭巧玲,杨祥胜.深海沉积物宏基因组文库中产蛋白酶克隆的筛选及性质分析.应用与环境生物学报,2009,15(4): 507-510. Guo Q L, Yang X S. Chin J Appl Environ Biol,2009,15(4): 507-510.
[13] Shuhaimi-Othman M, Pascoe D. Bioconcentration and depuration of copper, cadmium, and zinc mixtures by the freshwater amphipod Hyalella azteca. Ecotoxicology and Environmental Safety, 2007,66(1): 29-35.
[14] Isar J, Agarwal L, Saran S, et al. A statistical approach to study the interactive effects of process parameters on succinic acid production from Bacteroides fragilis. Anaerobe, 2007, 13 :50-56.
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