Research on Screening , Identification and Enzyme Producing Characteristics of Chitinase Producer

doi:Q93-3

China Biotechnology

2010, Vol. 30

Issue (08): 82-87 DOI: Q93-3

Research on Screening , Identification and Enzyme Producing Characteristics of Chitinase Producer

ZHANG Hai-tao,WANG Ting,TIAN Nan,LIU Xin-li

Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Institute of Light Industry, Jinan 250353, China

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Abstract

C65-2 strain with high chitinase producing ability was isolated from soil and identified it as Aspergillus fumigatus by observing its morphology character and measuring its 18S rDNA sequence. The chitinase producing conditions was optimized. The maximum activity could be up to 6.9 U/ml and the enzyme activity increased 210% than before. The study of enzymatic properties of the chitinase showed that the molecular weight was 20 Kda. Thermostable stability of the chitinase was not good and the enzyme activity reduced to 0 at 60 ℃ for 50 min. The optimal enzyme reaction temperature was 55 ℃ and the optimal pH of enzyme reaction was 7.0. The chitinase activity can be enhanced by Mg2+，Cu2+ and inhibited by Fe3+.

Key words： Chitin Chitinase Aspergillus fumigatus Enzyme producing characteristics

Received: 24 February 2010 Published: 25 August 2010

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	ZHANG Hai-Chao
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Cite this article:

ZHANG Hai-Chao, WANG Ting, TIAN Nan, LIU Xin-Li. Research on Screening , Identification and Enzyme Producing Characteristics of Chitinase Producer. China Biotechnology, 2010, 30(08): 82-87.

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https://manu60.magtech.com.cn/biotech/Q93-3 OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I08/82

［1］蒋挺大. 甲壳素. 北京:中国环境科学出版社, 1999. Jiang T D,Chitin.Beijing:China Environmental Science Press,1999.
［2］ Haki G D, Rakshit S K. Development in industrially important thermostable enzymes. Bioresource Technology ,2003 ,89 (1): 1734.
［3］ Carlini C R , Grossi D S , Maria F. Plant toxic proteins with insecticidal properties. Toxicon, 2002 ,40 (11) :15191539.
［4］林毅, 陈金辉. 芽孢杆菌几丁质酶及其在植物病虫害生物防治中的应用. 福建农业科技, 1998( 增刊): 3233. Lin Y,Chen J H. Fujian Agricultural Science and Technology,1998（supple）:3233.
［5］ Laura E V, Joseph O F. Comparison of methods for isolation of Mycobacterium avium complex DNA for use in PCR and RAPD fingerprinting. Microbiol Methods,1995 ,21(2):151161.
［6］ Collinge D B, Kragh K M, Mikkelsen J D, et al. Plant chitinases. The Plant Journal, 1993, 3 (1) : 31  40.
［7］ Schlumbrum A, Mauch F. Plant chitinases are potent inhibitors of fungal growth. Nature, 1986, 324 (4376) : 365 367.
［8］潘志强，黄惠琴，单群，等.Serratia sp PS2产几丁质酶发酵条件研究.工业微生物，2008，38（2）：40. Pan Z Q,Huang H Q,Shan Q, et al.Industrial Microbiology,2008，38（2）：40.
［9］苏广宇，李从发，高阳，等.产几丁质酶海洋细菌筛选及发酵条件优化.中国新药杂志，2008，17（17）：15031506. Su G Y, Li C F,Gao Y, et al. Chinese Journal of New Drugs, 2008,17（17):15031506.
［10］惠丰立，冯金荣，杨柯金，等.产几丁质酶酵母菌的筛选与鉴定. 东北林业大学报，2007，35（8）：6668. Hui F L,Feng J R,Yang K J, et al.Journal of Northeast Forestry University,2007,35(8):6668.
［11］王海东，陈飚，伦镜盛，等.产几丁质酶菌SWCH6的筛选鉴定及其产酶条件的优化研究. 微生物学通报，2008，35（5）：705711. Wang H D,Chen B,Lun J S, et al. Microbiology,2008,35(5):705711.
［12］杨水英，李振轮，青玲，等.产几丁质酶内生细菌的筛选及对烟草赤生病菌的抑制作用. 河南农业科学，2007,6:6669. Yang S Y,Li Z L,Qing L, et al. Journal of Henan Agricultural Sciences,2007,6:6669.
［13］杨水英，李振轮，青玲，等.产几丁质酶烟草内生菌的筛选及产酶条件研究. 西北农业学报，2007，16（4）：223227. Yang S Y,Li Z L,Qing L, et al. Acta Agriculturae Borealioccidentalis Sinica,2007,16(4):223227.
［14］邱立友,汪世山,余功德,等.几丁质酶产生菌的筛选及产酶条件的研究. 微生物学报，2000，27（4）：270272. Qiu L Y,Wang S S,Yu G D, et al. Microbiology,200,27(4):270272.
［15］田强，张彩，吴子健.几丁质酶产生菌的诱变育种及摇瓶发酵条件优化. 食品科学，2007，28（9）：405409. Tian Q,Zhang C,Wu Z J. Food Science,2007,28(9):405409.

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