|
|
|
| Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis as Achieved by Treatment with Chitin |
| LIU Ya-qing1, HOU Xiao-lun1, GUO Wei-ting1, CHANG Zhong-yi1, GAO Hong-liang1, BU Guo-jian2, LU Wei2, XIE Xiu-juan2, JIN Ming-fei1 |
1. Life Science College of East China Normal University, Shanghai 200241, China;
2. Taixing Dongsheng Food Science and Technology Limited Company, Taixing 225411, China |
|
|
|
Abstract The effects of chitin on the production of transglutaminase (TGase) in Streptoverticillium mobaraensis were tested. It indicated that enzyme activity had increased extremely significantly with the adding of 0.5% chitin compared with control, but 2% of chitin inhibit strains from producing TGase. Next, the mechanism about the enhancement of TGase production by chitin from four aspects,which was the pH changes during the process of metabolism, the production of TGase, the protein content and total nitrogen content in the fermented liquid were discussed. Studies show that chitin produce a certain stress for the growth of strain,thus stimulate the produce of secondary metabolites, so as to improve the production of TGase. Microscopic observation of strain fermentation process shows that it could be improving of the production of TGase by dispersing strains.
|
|
Received: 04 March 2015
Published: 25 June 2015
|
|
|
|
Cite this article:
LIU Ya-qing, HOU Xiao-lun, GUO Wei-ting, CHANG Zhong-yi, GAO Hong-liang, BU Guo-jian, LU Wei, XIE Xiu-juan, JIN Ming-fei. Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis as Achieved by Treatment with Chitin. China Biotechnology, 2015, 35(6): 40-45.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150607 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I6/40
|
|
|
|
[1] Yokoyama K, Keiichi U, Hiroe N, et al. Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis. Applied Microbiology and Biotechnology,2010, 87(6):2087-2096.
[2] Zotzel J, Keller P, Fuchsbauer H L. Transglutaminase from Streptomyces mobaraensis is activated by an endogenous metalloprotease. European Journal of Biochemistry, 2003,270(15): 3214-3222.
[3] Yokoyama K, Nio N, Kikuchi Y. Properties and applications of microbial transglutaminase. Applied Microbiology and Biotechnology, 2004,64(4):447-454.
[4] Junqua M, Duran R, Gancet C, et al. Optimization of microbial transglutaminase production using experimental designs. Applied Microbiology and Biotechnology, 1997,48(6): 730-734.
[5] 陈坚,李寅.发酵过程优化原理与实践.北京:化学工业出版社,2002. Cheng J, Li Y. Fermentation Process Optimization Theory and Practice. Beijing: Chemical Industry Press, 2002.
[6] 张程杰,堵国成,李寅,等.添加羟胺诱导Streptomyces hygroscopicus产谷氨酰胺转胺酶.过程工程学报, 2005(4):434-437. Zhang C, Du G C, Li Y, et al. Induced microbial transglutaminase accumulation by Streptomyces hygroscopicus due to addition of hydroxylamine. The Chinese Journal of Process Engineering, 2005(4): 434-437.
[7] 程力,张东旭,堵国成,等.添加胰蛋白酶促进Streptomyces hygroscopicus CCTCC M203062产谷氨酰胺转胺酶.中国生物工程杂志,2010 (7): 53-58. Cheng L, Zhang D X, Du G C, et al. Improvement on the activity of microbial transglutaminase with Streptomyces hygroscopicus by the addition of surfactant CTAB. Chin Journal of Biotechnology, 2010,7:53-58.
[8] Zhang L, Han X. Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved by treatment with excessive MgCl2. Applied Microbiology and Biotechnology, 2012,93(6):2335-2343.
[9] Kean T, Thanou M. Biodegradation, biodistribution and toxicity of chitosan. Advanced Drug Delivery Reviews, 2010,62(1):3-11.
[10] Kim S K, Rajapakse N. Enzymatic production and biological activities of chitosan oligosaccharides (COS): a review. Carbohydrate Polymers, 2005,62(4):357-368.
[11] Marin L, Ailincai D, Mares M, et al. Imino-chitosan biopolymeric films. Obtaining, self-assembling, surface and antimicrobial properties. Carbohydrate Polymers, 2015,117:762-770.
[12] Zhong Z, Xing R, Liu S,et al. Synthesis of acylthiourea derivatives of chitosan and their antimicrobial activities in vitro. Carbohydrate Research, 2008,343(3):566-570.
[13] Grossowicz N, Wainfan E, Borek E, et al. The enzymatic formation of hydroxamic acids from glutamine and asparagine. The Journal of Biological Chemistry, 1950,187(1): 111-125.
[14] Martin J, Liras P. Engineering of regulatory cascades and networks controlling antibiotic biosynthesis in Streptomyces. Current Opinion in Microbiology, 2010,13(3):263-273.
[15] Yang F, Yang M, Cheng S H. A novel method to enhance the mycelia production of Ganoderma lucidum in submerged cultures by polymer additives and agitation strategies. Journal of the Taiwan Institute of Chemical Engineers, 2009,40(2):148-154.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
