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| Ethidium Bromide-Induced Transient Demyelination Increases the Efficiency of Retrograde PRV Transduction |
LIU Fa-dong1,DUAN Hong-mei2,HAO Peng2,ZHAO Wen2,GAO Yu-dan2,YANG Zhao-yang2,3,LI Xiao-guang1,2,3,**( ) |
1 Beijing Key Laboratory for Biomaterials and Neural Regeneration, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China
2 Department of Neurobiology, Capital Medical University, Beijing 100069, China
3 Beijing International Cooperation Bases for Science and Technology on Biomaterials and Neural Regeneration,Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100083, China |
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Abstract Objective: To improve the efficiency of PRV transduction by ethidium bromide (EB) -induced transient demyelination.Methods: 18 adult Wistar rats were randomly divided into muscle group, NS group and EB group(n=6).In the muscle group, 2μl PRV with a titer of 2×10 9 was injected into the tibial anterior muscle and gastrocnemius muscle. NS group was injected with 2μl normal saline (NS) to the sciatic nerve, and 2μl PRV with a titer of 2×10 9 was injected to the same site one week later. In the EB group, 2μl 0.1% EB was injected into the sciatic nerve, and 2μl PRV with a titer of 2×10 9 was injected into the same position one week later. Five days later, perfusion samples were taken and frozen sections were made to observe the infection of neurons at all levels. Methods: A large number of L4-L5 spinal anterior horn neurons, dorsal root ganglion (DRG)neurons, T8 spinal intermediate neurons, C4 spinal intermediate neurons, medulla oblongata, midbrain and cerebral cortex of rats in EB group were labeled by PRV.A small number of neurons at all levels were labeled by PRV in the muscle group and the NS group.Conclusion: EB- induced sciatic nerve demyelination can enhance the reverse transduction efficiency of PRV.
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Received: 10 May 2019
Published: 15 January 2020
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Corresponding Authors:
Xiao-guang LI
E-mail: lxgchina@sina.com
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