信号肽在大肠杆菌分泌系统中的研究与应用进展

doi:10.13523/j.cb.2101018

中国生物工程杂志

2021, Vol. 41

Issue (5): 87-93 DOI: 10.13523/j.cb.2101018

综述

信号肽在大肠杆菌分泌系统中的研究与应用进展

何若昱^1,²,林福玉²,高向东^1,^*(

),刘金毅^2,^*(

)

1 中国药科大学生命科学与技术学院南京 210009
2 北京三元基因药业股份有限公司北京 102600

Research and Application Progress of Signal Peptides in Escherichia coli Secretion Systems

HE Ruo-yu^1,²,LIN Fu-yu²,GAO Xiang-dong^1,^*(

),LIU Jin-yi^2,^*(

)

1 School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
2 Beijing Tri-Prime Genetic Engineering Co., Ltd, Beijing 102600, China

全文: PDF(429 KB) HTML

摘要：

大肠杆菌以其明显的优势成为表达重组蛋白常用的系统,但是大肠杆菌本身不具备细胞内形成二硫键的氧化条件和分子机制,而且高水平表达时常容易聚集形成包涵体,限制了其使用,改善这一缺点的重要方法是通过信号肽实现蛋白质的分泌表达。信号肽一般存在于分泌蛋白的氨基端,能够引导蛋白质通过大肠杆菌中的Sec或/和Tat系统分泌至周质空间。简要概述了大肠杆菌中两种跨膜分泌系统和信号肽的结构,并结合近年来常用6种信号肽的研究与应用进展,阐述了信号肽在使用中存在的问题及改进措施。旨在为研究者合理选择信号肽、优化重组蛋白的表达提供更多可用的信息与策略。

关键词： 信号肽; 大肠杆菌; 分泌系统

Abstract:

Escherichia coli has become a commonly used system for expressing recombinant proteins due to its obvious advantages. However, E. coli does not have the oxidative conditions and molecular mechanisms for the formation of disulfide bonds in the cytoplasm, and high-level expression often tends to aggregate to form inclusion bodies, which limits its use. Signal peptides to guide proteins secretion can overcome this shortcoming. The proteins with signal peptides are secreted by the Sec or/and Tat systems in E. coli. The E. coli secretion systems and the structure of signal peptides were summarized. Meanwhile, the research and application progress of six commonly used signal peptides in recent years were introduced. Finally, the problems and improvement measures in the application of signal peptides were summarized,which would provide more useful information and strategies for researchers to select signal peptides reasonably and optimize the expression of recombinant proteins.

Key words: Signal peptide Escherichia coli Secretion system

收稿日期: 2021-01-14 出版日期: 2021-06-01

ZTFLH:

Q816

通讯作者: 高向东,刘金毅 E-mail: xdgao@cpu.edu.cn;liujinyi@triprime.com

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引用本文:

何若昱,林福玉,高向东,刘金毅. 信号肽在大肠杆菌分泌系统中的研究与应用进展[J]. 中国生物工程杂志, 2021, 41(5): 87-93.

HE Ruo-yu,LIN Fu-yu,GAO Xiang-dong,LIU Jin-yi. Research and Application Progress of Signal Peptides in Escherichia coli Secretion Systems. China Biotechnology, 2021, 41(5): 87-93.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2101018 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I5/87

表1 大肠杆菌系统中常用的6种信号肽

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