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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2019, Vol. 39 Issue (9): 41-49    DOI: 10.13523/j.cb.20190906
研究报告     
山西省健康成年人淋巴细胞亚群正常参考值范围 *
惠怡华1,王海娜2,戚宇锋1,曹雪玲3,管雪梅3,段静静2,段轶鋆2,王艳峰2,苏文2,**()
1 山西医科大学第二临床医学院 太原 030001
2 山西医科大学附属肿瘤医院 山西省肿瘤研究所 太原 030013
3 山西医科大学附属肿瘤医院 太原 030013
Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province
HUI Yi-hua1,WANG Hai-na2,QI Yu-feng1,CAO Xue-ling3,GUAN Xue-mei3,DUAN Jing-jing2,DUAN Yi- jun2,WANG Yan- feng2,SU Wen2,**()
1 Second Hospital of Shanxi Medical University, Taiyuan 030001, China
2 Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China
3 Health Examination Center, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China
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摘要：

目的: 建立山西省健康成人外周血淋巴细胞亚群的正常参考值范围,为机体免疫状态的分析和肿瘤患者的免疫评估提供理论依据。方法: 选取山西省1 238例健康成人体检人群,采用流式细胞术测定外周血淋巴细胞亚群的绝对计数和相对计数。结果： 确定了健康成人外周血淋巴细胞表达水平,并发现CD3 +T细胞相对计数和绝对计数、CD4 +T细胞相对计数、CD8 +T细胞相对计数和绝对计数、NK细胞相对计数和绝对计数、CD19细胞相对计数和绝对计数、CD4/CD8比值在不同年龄组间存在显著差异性(P<0.05);不同性别之间CD8 +T细胞相对计数、CD4 +T细胞绝对计数和CD19细胞绝对计数无统计学意义,CD3 +T细胞、CD8 +T细胞、NK细胞相对计数和绝对计数、CD4 +T细胞、CD19细胞相对计数均存在显著差异(P<0.05)。 结论： 初步建立了山西省健康成年人外周血淋巴细胞亚群参考值范围,为机体免疫功能的评价和肿瘤免疫治疗、诊断提供了参考依据。
关键词： 外周血淋巴细胞亚群;  正常参考值范围;  流式细胞术;  山西省    
Abstract:

Objective: To establish normal reference range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province and provide theoretical basis for studying immunity status and assessing immune function in cancer patients.Methods: Selected 1 238 healthy adults in Shanxi Province and used six-color monoclonal antibody to detect and analysis absolute and percentage counts of peripheral blood lymphocyte subsets.Results: The expression of peripheral blood lymphocytes levels was determined, and it was found that CD3 +T、CD4 +T、CD8 +T、NK、CD19 cell percentage and absolute counts and CD4/CD8 ratio dissimilarity with a P value <0.05 was considered statistically significant ;and there was no statistical significance in CD8 + T cell percentage counts、CD4 +T cell and CD19 cell absolute counts between different genders,CD3 +T cell、CD8 +T cell、NK cell percentage and absolute counts、CD4 +T cell、CD19 cell percentage counts dissimilarity with a P value <0.05 was considered statistically significant. Conclusion: The refe -rence range of peripheral blood lymphocyte subsets in healthy adults in Shanxi Province was preliminarily established ,which provided reference for the evaluation of immunity function, tumor immunotherapy and diagnosis.

Key words: T lymphocyte subsets    Normal reference values    Flow cytometry    ShanXi Province
收稿日期: 2019-08-21 出版日期: 2019-09-20
ZTFLH:  Q26  
基金资助: * 国家自然科学基金项目(1272696);山西省优秀人才科技创新项目(201705D211021)
通讯作者: 苏文     E-mail: suwen30@yahoo.com
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惠怡华
王海娜
戚宇锋
曹雪玲
管雪梅
段静静
段轶鋆
王艳峰
苏文

引用本文:

惠怡华,王海娜,戚宇锋,曹雪玲,管雪梅,段静静,段轶鋆,王艳峰,苏文. 山西省健康成年人淋巴细胞亚群正常参考值范围 *[J]. 中国生物工程杂志, 2019, 39(9): 41-49.

HUI Yi-hua,WANG Hai-na,QI Yu-feng,CAO Xue-ling,GUAN Xue-mei,DUAN Jing-jing,DUAN Yi- jun,WANG Yan- feng,SU Wen. Normal Reference Range of Lymphocyte Subsets in Healthy Adults in Shanxi Province. China Biotechnology, 2019, 39(9): 41-49.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190906    或    https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I9/41

T淋巴细胞亚型 相对计数(%) 绝对计数(个/μl) CD4/CD8 ratio
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
x? ±s 68.70±9.55 28.62±8.48 36.89±7.99 19.71±9.33 10.87±4.34 1 492.27±530.71 626.80±303.86 796.22±301.96 431.41±279.11 236.39±132.99 1.44±0.63
95%参考值范围 62.77~75.59 22.42~33.45 21.23~52.55 12.91~25.02 7.89~13.26 1 129.16~1 798.46 425.82~764.89 590.34~969.80 253.83~534.65 155.20~290.96 1.01~1.76
表1  1 238例健康成人外周血T淋巴细胞亚群参考值范围
年龄组和性别
(n=例数)		 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
≤39(n=400)
男(n=93) 54.23~83.47 15.47~46.67 20.55~44.53 4.84~34.95 7.87~12.93 701.53~2 536.28 180.59~1 290.54 615.10~878.51 306.55~573.42 170.15~297.50 0.79~1.38
女(n=307) 66.51~77.36 23.14~34.43 25.72~50.79 10.78~20.90 8.38~13.07 1 224.68~1 841.61 456.25~764.84 633.79~1 000.84 202.96~460.34 167.01~301.76 1.03~1.77
40-59(n=560)
男(n=194) 63.01~73.36 21.8~,33.45 19.41~53.32 18.73~32.23 7.98~13.67 1 239.35~1 934.23 465.85~834.33 634.00~1 090.10 298.86~622.68 171.23~333.30 0.98~1.81
女(n=366) 63.97~76.45 22.28~31.82 33.62~43.94 12.52~23.38 8.33~13.35 1 101.68~1 627.46 393.35~675.24 587.77~947.39 222.69~460.16 153.47~265.50 1.15~1.83
≥60(n=278)
男(n=96) 42.14~87.76 10.18~52.25 14.06~48.93 3.68~43.29 5.00~11.89 994.71~1 820.33 431.13~908.25 499.31~912.97 358.85~732.63 99.33~238.96 0.71~1.55
女(n=182) 41.77~87.76 20.35~32.75 19.06~52.31 16.11~29.34 7.69~13.48 906.27~1 014.75 368.91~737.38 501.12~909.24 274.25~637.77 129.79~288.65 0.97~1.80
表2  不同年龄段不同性别的健康成年人95%参考值范围
性别(n=例数) 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
女性(n=855) 69.44±9.45 28.16±8.07 38.10±7.48 18.75±8.79 11.10±4.31 1 445.45±500.20 588.22±265.67 790.28±288.54 395.88±259.02 232.79±135.63 1.49±0.61
男性(n=383) 67.03±9.58 29.65±9.27 34.21±8.45 21.83±10.15 10.37±4.37 1 596.78±580.41 712.94±364.15 809.49±329.95 510.74±305.18 244.43±126.70 1.32±0.65
P <0.001 0.14 <0.001 <0.001 0.004 <0.001 <0.001 0.69 <0.001 0.132 0.001
表3  不同性别健康成人的外周血T淋巴细胞亚群比较
年龄组(n=例数) 相对计数(%) 绝对计数(个/μl) CD4/CD8 比值
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
≤39(n=400) 71.14±7.77 29.61±7.82 36.93±6.77 17.35±7.8 10.91±3.47 1 581.18±492.46 662.48±280.78 818.35±276.05 391.23±236.16 244.49±114.87 1.39±0.52
40~59(n=560) 69.33±8.97 27.72±8.02 38.18±8.05 18.96±8.60 11.10±4.04 1 490.18±527.25 597.74±281.73 818.31±320.45 410.11±258.78 237.06±115.52 1.53±0.67
≥60(n=278) 63.90±11.22 29.00±10.02 34.24±8.84 24.60±10.91 10.37±5.80 1 368.53±566.27 634.00±367.42 720.19±287.57 532.15±344.91 223.39±181.04 1.36±0.69
P <0.001 <0.001 0.004 <0.001 <0.001 <0.001 <0.001 0.69 <0.001 0.003 <0.001
表4  不同年龄段健康成人外周血T淋巴细胞亚群比较
年龄组 P CD4/CD8 比值
相对计数(%) 绝对计数(个/μl)
CD3 CD8 CD4 NK CD19 CD3 CD8 CD4 NK CD19
27-39 40-59 0.014 0.002 0.086 0.037 1.000 0.001 <0.001 0.994 0.936 0.479 0.001
60-90 <0.001 0.749 <0.001 <0.001 0.011 <0.001 0.008 <0.001 0.011 <0.001 1.000
40-59 60-90 <0.001 0.255 <0.001 <0.001 0.004 0.001 1.000 <0.001 0.004 0.008 <0.001
表5  各年龄组间两两比较P值结果
图1  外周血淋巴细胞亚群在不同年龄段的变化趋势比较
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