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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2019, Vol. 39 Issue (7): 56-64    DOI: 10.13523/j.cb.20190708
技术与方法     
小球藻与固氮菌Mesorhizobium sp.共培养对小球藻生长和油脂积累的促进效果 *
卫治金,李晓,王皓楠,尹永浩,郗丽君,葛保胜()
中国石油大学(华东)生物工程与技术中心 青岛 266580
Enhanced Biomass Production and Lipid Accumulation by Co-cultivation of Chlorella vulgaris with Azotobacter Mesorhizobium sp.
Zhi-jin WEI,Xiao LI,Hao-nan WANG,Yong-hao YIN,Li-jun XI,Bao-sheng GE()
Center for Bioengineering and Biotechnology,China University of Petroleum (East China),Qingdao 266580,China
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摘要：

微藻油脂不仅可以作为功能油脂,同时也是生产生物柴油的重要原料之一。为解决微藻生长与油脂积累之间的矛盾,利用藻菌共培养技术在缺氮条件下将无菌小球藻与细菌以不同初始比例进行共培养,通过测定藻细胞生物量、油脂含量和脂肪酸比例等来研究藻菌共培养对小球藻生长和油脂积累的影响。结果表明,在小球藻与固氮菌B2.3 70∶1(V/V)共培养体系中,小球藻的生物量和油脂含量较同样条件下单独培养小球藻有了显著提高。其生物量最高可达1.68g/L、总脂含量为45.2%、总脂产率为75.94 mg/(L·d)、中性脂含量为23.0%及中性脂产率为38.65mg/(L·d),其生物量和油脂含量分别较单独小球藻培养时提高了66.3%和47.7%。同时细菌的加入显著提高了藻细胞内C18∶1脂肪酸的比例。结论表明,通过藻菌共培养技术能够有效提高微藻生物油脂的质量和产量,具有较好的实际利用价值。
关键词： 藻菌共培养;  小球藻;  生物量;  油脂积累    
Abstract:

Microalgal oil is an important functional oil, but also one of the major sources for bio-diesel production. In order to solve the contradiction of algae growth and lipid accumulation, the Chlorella vulgaris were co-cultured with azotobacter at different initial ratio under nitrogen source deficiency conditions, and the impact of consortium system growth, lipid accumulation based on the dry weight, lipid content, and fatty acids composition in the incubation period were evaluated. The results showed that the biomass and lipid content of algae were significantly higher than that of the pure culture of axenic C.vulgaris when C. vulgaris and B2.3 strains were inoculated at the ratio of 70∶1. The biomass, lipid content, lipid productivity, neutral lipid content and neutral lipid productivity of algae in the co-cultures were 1.68g/L, 45.2%, 75.94mg/(L·d), 23.0% and 38.65mg/(L·d), respectively. And the biomass concentration and lipid content in the co-culture system were 66.3% and 47.7% higher than that of the axenic pure algal cultures, respectively. Furthermore, the consortium system significantly increased the proportion of C18∶1 fatty acids. It was indicated that the co-cultivation of algae-bacteria system can effectively contribute to the quality and quantity of microalgal bio-oil, and has great potential for production of bio-diesel.
Key words: Co-culture    Chlorella vulgaris    Biomass    Lipid accumulation
收稿日期: 2018-12-06 出版日期: 2019-08-05
ZTFLH:  Q819  
基金资助: * 国家重点研发计划资助项目(2016YFE0106700)
通讯作者: 葛保胜     E-mail: gebaosheng@upc.edu.cn
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卫治金
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引用本文:

卫治金,李晓,王皓楠,尹永浩,郗丽君,葛保胜. 小球藻与固氮菌Mesorhizobium sp.共培养对小球藻生长和油脂积累的促进效果 *[J]. 中国生物工程杂志, 2019, 39(7): 56-64.

Zhi-jin WEI,Xiao LI,Hao-nan WANG,Yong-hao YIN,Li-jun XI,Bao-sheng GE. Enhanced Biomass Production and Lipid Accumulation by Co-cultivation of Chlorella vulgaris with Azotobacter Mesorhizobium sp.. China Biotechnology, 2019, 39(7): 56-64.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190708    或    https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I7/56

图1  小球藻与Mesorhizobium sp.共培养10d后小球藻生物量和比生长速率的变化
图2  小球藻与Mesorhizobium sp.共培养10d后小球藻总脂含量及产率的变化
图3  小球藻与Mesorhizobium sp.共培养10d后小球藻中性脂含量及产率的变化
图4  小球藻与Mesorhizobium sp.共培养10d后小球藻脂肪酸比例的变化
图5  小球藻与Mesorhizobium sp.共培养10d胞外多聚物中蛋白质浓度的变化
图6  小球藻与Mesorhizobium sp.共培养10d后胞外多聚物中多糖浓度的变化
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