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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (3): 33-40    DOI: 10.13523/j.cb.20180305
研究报告     
中间锦鸡儿CibHLH027基因的克隆和功能研究*
杨天瑞1*,李娜1*,张秀娟2,杨杞1,王瑞刚1,李国婧1**()
1 内蒙古农业大学生命科学学院 呼和浩特 010018
2 内蒙古生物技术研究院 呼和浩特 010010
Cloning and Functional Characterization of CibHLH027 Involved in Leaf Senescence from Caragana intermedia
Tian-rui YANG1*,Na LI1*,Xiu-juan ZHANG2,Qi YANG1,Rui-gang WANG1,Guo-jing LI1**()
1 College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China
2 Inner Mongolia Institute of Biotechnology, Hohhot　010010,China
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摘要：

叶片衰老是植物生长发育的最后阶段,黑暗、干旱和极端温度等会加速叶片衰老过程,严重影响植物的生长发育和作物产量。bHLH(basic helix-loop-helix)属于转录调控因子中的一个大家族,因具有保守性较高的bHLH结构域而得名,广泛存在于真核生物界。在中间锦鸡儿(Caragana intermedia)转录组数据库中得到一条候选bHLH基因全长序列,将其命名为CibHLH027。正常和黑暗诱导的情况下,CibHLH027-OE株系较WT相比叶片衰老明显,并且黑暗处理后叶绿素含量下降、离子渗透率增大、细胞膜受损严重,说明CibHLH027参与了叶片衰老调控过程。
关键词： 中间锦鸡儿;  CibHLH027;  黑暗;  叶片衰老    
Abstract:

Leaf senescence is the last phase of plant development, environmental factors such as dark, drought, extreme temperature can promote leaf senescence, which may influence the plant growth and crop yield. Basic/helix-loop-helix(bHLH) transcription factors (TFs)belong to a superfamily of transcriptional regulators, it is named bHLH for its highly conserved bHLH domain, and widely exists in the eukaryotes. A candidate bHLH encoding gene, CibHLH027, was screened from the transcriptome library of Caragana intermedia. Compared with wild type, CibHLH027-OE promoted leaf senescence under both light and darkness, with reduced chlorophyll content, increased ion leakage and cell death under darkness, indicates that CibHLH027 is involved in leaf senescence.
Key words: Caragana    intermedia    CibHLH027    Darkness    Leaf    senescence
收稿日期: 2017-09-28 出版日期: 2018-04-04
ZTFLH:  Q78  
基金资助: 国家自然科学基金(31360169);内蒙古自治区科技创新团队(201503004)
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杨天瑞
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引用本文:

杨天瑞,李娜,张秀娟,杨杞,王瑞刚,李国婧. 中间锦鸡儿CibHLH027基因的克隆和功能研究*[J]. 中国生物工程杂志, 2018, 38(3): 33-40.

Tian-rui YANG,Na LI,Xiu-juan ZHANG,Qi YANG,Rui-gang WANG,Guo-jing LI. Cloning and Functional Characterization of CibHLH027 Involved in Leaf Senescence from Caragana intermedia. China Biotechnology, 2018, 38(3): 33-40.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180305    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I3/33

图1  CibHLH027基因PCR产物及CibHLH027-pCambia1302、CibHLH027-pCanG载体的酶切验证
图2  qRT-PCR检测CibHLH027对非生物胁迫的响应情况
图3  CibHLH027-GFP在拟南芥原生质体中的亚细胞定位
图4  CibHLH027-OE转基因株系的鉴定
图5  CibHLH027叶片衰老现象
图6  黑暗诱导下CibHLH027过表达株系与野生型衰老表型比较
图7  黑暗处理后CibHLH027-OE株系和野生型拟南芥叶片离子渗透和细胞死亡比较
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