技术与方法 |
|
|
|
|
莱茵衣藻LZTFL1蛋白的多克隆抗体制备及应用 |
刘雁霞, 樊振川 |
天津科技大学大健康生物技术研究所 天津市大健康生物技术国际联合研究中心 天津科技大学食品工程与生物技术学院 天津 300457 |
|
Polyclonal Antibody Preparation and Application of C.reinhardtii LZTFL1 Protein |
LIU Yan-Xia, FAN Zhen-Chuan |
Institute of Health Biotechnology, International Collaborative Research Center for Health Biotechnology, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China |
[1] |
Merchant Sabeeha S, Prochnik Simon E, Vallon O, et al. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science, 2007, 318(5848):245-250.
|
[2] |
Badano J L, Mitsuma N, Beales P L, et al. The ciliopathies:an emerging class of human disorders. Annu Rev Genomics Hum Genet, 2006, 7:125-148.
|
[3] |
Aldahmesh M A, Li Y, Alhashem A, et al. IFT27, encoding a small GTPase component of IFT particles, is mutated in a consanguineous family with Bardet-Biedl syndrome. Hum Mol Genet, 2014, 23(12):3307-3315.
|
[4] |
Schaefer E, Lauer J, Durand M, et al. Mesoaxial polydactyly is a major feature in Bardet-Biedl syndrome patients with LZTFL1(BBS17) mutations. Clin Genet, 2014, 85(5):476-481.
|
[5] |
Forsythe E, Beales P L. Bardet-Biedl syndrome. Eur J Hum Genet, 2013, 21(1):8-13.
|
[6] |
Marion V, Stutzmann F, Gerard M, et al. Exome sequencing identifies mutations in LZTFL1, a BBSome and smoothened trafficking regulator, in a family with Bardet——Biedl syndrome with situs inversus and insertional polydactyly. J Med Genet, 2012, 49(5):317-321.
|
[7] |
Marion V, Mockel A, De Melo C, et al. BBS-induced ciliary defect enhances adipogenesis, causing paradoxical higher-insulin sensitivity, glucose usage, and decreased inflammatory response. Cell Metab, 2012, 16(3):363-377.
|
[8] |
Zhang Q, Nishimura D, Seo S, et al. Bardet-Biedl syndrome 3(Bbs3) knockout mouse model reveals common BBS-associated phenotypes and Bbs3 unique phenotypes. Proc Natl Acad Sci U S A, 2011, 108(51):20678-20683.
|
[9] |
Yen H J, Tayeh M K, Mullins R F, et al. Bardet-Biedl syndrome genes are important in retrograde intracellular trafficking and Kupffer's vesicle cilia function. Hum Mol Genet, 2006, 15(5):667-677.
|
[10] |
Mykytyn K, Mullins R F, Andrews M, et al. Bardet-Biedl syndrome type 4(BBS4)-null mice implicate Bbs4 in flagella formation but not global cilia assembly. Proc Natl Acad Sci USA, 2004, 101(23):8664-8669.
|
[11] |
Kim J C, Badano J L, Sibold S, et al. The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression. Nat Genet, 2004, 36(5):462-470.
|
[12] |
Jiang J, Promchan K, Jiang H, et al. Depletion of BBS protein LZTFL1 affects growth and causes retinal degeneration in mice. J Genet Genomics, 2016, 43(6):381-391.
|
[13] |
Kiss H, Kedra D, Kiss C, et al. The LZTFL1 gene is a part of a transcriptional map covering 250 kb within the common eliminated region 1(C3CER1) in 3p21.3. Genomics, 2001, 73(1):10-19.
|
[14] |
Eguether T, San Agustin J T, Keady B T, et al. IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment. Dev Cell, 2014, 31(3):279-290.
|
[15] |
Seo S, Zhang Q, Bugge K, et al. A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened. PLoS Genet, 2011, 7(11):e1002358.
|
[16] |
Wang Z, Fan Z C, Williamson S M, et al. Intraflagellar transport (IFT) protein IFT25 is a phosphoprotein component of IFT complex B and physically interacts with IFT27 in Chlamydomonas. PLoS One, 2009, 4(5):e5384.
|
[17] |
Fan Z C, Bird R C. An alternative -1/+2 open reading frame exists within viral N(pro)(1-19) region of bovine viral diarrhea virus SD-1. Virus Res, 2012, 163(1):341-351.
|
[18] |
董彬, 吴淞, 王晶, 等. 莱茵衣藻纤毛内运送蛋白IFT27的原核表达纯化及多克隆抗体制备. 生物技术学报, 2016, 26(6):532-538. Dong B, Wu S, Wang J, et al. Prokaryotic expression,purification and polyclonal antibody preparation of the Chlamydomonas Reinhardtii intraflagellar transport protein (IFT27). Biotechnology, 2016, 26(6):532-538.
|
[19] |
任海月,董彬,樊振川,等. 莱茵衣藻纤毛内运输蛋白IFT46的原核表达纯化及其多克隆抗体的制备. 生物工程学报, 2016, 32(8):1124-1132. Ren H Y, Dong B, Fan Z C, et al. Prokaryotic expression and purification of Chlamydomonas reinhardtii intraflagellar transport protein 46(IFT46) and preparation of polyclonal antibody. Chinese Journal of Biotechnology, 2016, 32(8):1124-1132.
|
[20] |
Valcourt U, Gouttenoire J, Aubert-Foucher E, et al. Alternative splicing of type Ⅱ procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-beta1. FEBS Lett, 2003, 545(2-3):115-119.
|
[21] |
田伟,董彬,李振芳,等. 莱茵衣藻IFT139蛋白抗原的原核表达、纯化及多克隆抗体的制备.天津科技大学学报, 2016, 31(8):27-33. Tian W, Dona B, Li Z F, et, al. Prokaryotic expression,purification and polyclonal antibody preparation of Chlamydomonas reinhardtii IFT139 protein antigen. Journal of Tianjin University of Science & Technology, 2016, 31(8):27-33.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|