胭脂萝卜花青素提取及对NCI-N87细胞增殖侵袭的影响

doi:10.13523/j.cb.20171114

中国生物工程杂志

2017, Vol. 37

Issue (11): 101-108 DOI: 10.13523/j.cb.20171114

技术与方法

胭脂萝卜花青素提取及对NCI-N87细胞增殖侵袭的影响

梁姗, 蒋子川, 冯均

长江师范学院生命科学与技术学院重庆 408100

The Effect on NCI-N87 Cell Proliferation and Invasion by Carmine Radish Anthocyanin

LIANG Shan, JIANG Zi-chuan, FENG Jun

College of Life & Tech, Yangtze Normal University, Chongqing 408100, China

全文: PDF(962 KB) HTML

摘要： 为研究胭脂萝卜花青素的提取工艺，考察了液料比、浸提温度、浸提时间、超声功率、超声时间对花青素提取率的影响。采用MTT实验、细胞侵袭实验研究了花青素对人胃癌细胞NCI-N87增殖、侵袭的作用，进一步用免疫印迹分析其对HER2信号通过的影响。结果表明，结合响应面分析得出胭脂萝卜花青素最佳提取工艺为：以1%盐酸乙醇为提取剂，液料比10：1，40℃浸提2h，400W超声波破碎15min，在此条件下，花青素的最高提取量为3.92mg/g。30μg/ml的胭脂萝卜花青素能显著抑制NCI-N87细胞的增殖和侵袭能力，同时抑制HER2蛋白及Akt的磷酸化水平。

关键词： 细胞增殖; 胭脂萝卜; 花青素; 细胞侵袭

Abstract: To explore the extraction technology of anthocyanin from carmine radish, five single factors were studied including solid-liquid ratio, digestion temperature, digestion time, ultrasonic power, and ultrasonic power. MTT and Transwell^TM were used to discuss the proliferation, invasion ability of human gastric carcinoma cells(NCI-N87)which effect by anthocyanin from carmine radish. Furthermore, Western blot used to analyze the HER2 pathway. The optimum extraction technology was determined based on response surface analysis:1% acid-ethanol as extracting agent, 1:10 solid-liquid ratio, digestion 2h at 40℃ with 400W ultrasonic disruption for 15min. Under this condition, anthocyanin extraction content was 3.92mg/g. 30μg/ml anthocyanin from carmine radish inhibited NCI-N87 cell proliferating and invasion ability, decreased HER and p-Akt expression.

Key words: Carmine radish Anthocyanin Cell proliferation Cell invasion

收稿日期: 2017-07-05 出版日期: 2017-11-15

ZTFLH:

Q813

基金资助: 重庆市教委基金资助项目（KJ1601207）

通讯作者: 梁姗 E-mail: vmm420@hotmail.com

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引用本文:

梁姗, 蒋子川, 冯均. 胭脂萝卜花青素提取及对NCI-N87细胞增殖侵袭的影响[J]. 中国生物工程杂志, 2017, 37(11): 101-108.

LIANG Shan, JIANG Zi-chuan, FENG Jun. The Effect on NCI-N87 Cell Proliferation and Invasion by Carmine Radish Anthocyanin. China Biotechnology, 2017, 37(11): 101-108.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20171114 或 https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I11/101

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