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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (12): 82-90    DOI: 10.13523/j.cb.20181211
综述     
压电式微喷技术在细胞打印领域的应用*
孙怀远1,**(),宋晓康2,廖跃华1,李晓欧1
1 上海健康医学院医疗器械学院 上海 201318
2 上海理工大学医疗器械与食品学院 上海 200093
The Application of Piezoelectric Micro-jetting Technology in the Field of Cell Bioprinting
SUN Huai-yuan1,**(),SONG Xiao-kang2,LIAO Yue-hua1,LI Xiao-ou1
1 Medical Instrument College, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China;
2 College of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
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摘要：

细胞获取和培养的过程是组织工程构建的重要环节;3D生物打印技术可以支撑组织工程的发展。通过“声波”使微量流体在惯性力和流体粘性力相互作用下实现脉冲流动的压电式微喷技术应用于细胞打印领域属于新兴工艺,具有精度高、效率高、成本低等特征。在介绍压电式微喷技术系统和原理基础上,分析了压电驱动方式、压电参数、脉冲驱动电压波形、生物细胞油墨对细胞打印的影响;给出了压电式微喷技术在高存活率细胞获取、高效率构建细胞三维组织方面的应用研究案例;总结了其在细胞打印领域的应用现状,并指出研究方向及意义。
关键词： 微喷技术;  细胞打印;  组织工程    
Abstract:

The process of cell acquisition and culture is an important part of tissue engineering construction. Three-dimensional bioprinting technology can support the development of tissue engineering. Piezoelectric micro-jetting realize pulsating flow of trace fluid under the interaction of the inertial force and fluid viscous force by “sound wave”, its application in the field of cell bioprinting belongs to the emerging technology, and it has the characteristics of high precision, high efficiency as well as low cost. Based on the introduction of micro-jetting technology system and principle, the effects of piezoelectric drive mode, piezoelectric parameters, pulse drive voltage waveform and bio-cell ink on cell bioprinting were analyzed, the cases studied of piezoelectric micro-jetting technology in high livability cell acquisition and efficient construction of cell three-dimensional tissue were provided, its application status in the field of cell bioprinting and research direction as well as significance were summarized.
Key words: Micro-jetting technology    Cell bioprinting    Tissue Engineering
收稿日期: 2018-07-30 出版日期: 2019-01-10
ZTFLH:  Q819  
基金资助: *上海市生物医学工程学科建设项目(E1-2601-17-201002)
通讯作者: 孙怀远     E-mail: shy62123@163.com
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引用本文:

孙怀远,宋晓康,廖跃华,李晓欧. 压电式微喷技术在细胞打印领域的应用*[J]. 中国生物工程杂志, 2018, 38(12): 82-90.

SUN Huai-yuan,SONG Xiao-kang,LIAO Yue-hua,LI Xiao-ou. The Application of Piezoelectric Micro-jetting Technology in the Field of Cell Bioprinting. China Biotechnology, 2018, 38(12): 82-90.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20181211    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I12/82

图1  压电式微喷打印系统原理图
图2  压电喷头示意图
图3  压电驱动方式对比
图4  双极梯形波驱动电压
生物油墨打印细胞研究目的实验结果参考文献
聚乙烯吡咯烷成纤维细胞聚合物浓度对Z值和细胞打印稳定性的影响。通过改变聚合物浓度调节Z值可以提高细胞打印稳定性和细胞存活率。[16]
海藻酸钠和聚苯乙烯混合液成纤维细胞生物油墨浓度对打印细胞存活率的影响。生物油墨浓度降低,打印细胞存活率相应提高。[32]
聚蔗糖和磷酸盐缓冲溶液乳腺癌细胞生物油墨流变特性对细胞打印过程的影响。改善生物油墨流变性可以降低细胞聚集、沉积现象。[33-36]
无内毒素低酰基结冷胶悬浮液小鼠成肌细胞生物油墨流变特性对打印细胞存活率的影响。生物油墨流变性好,则打印的细胞存活率高。[37]
表1  几种生物油墨对细胞打印的影响
细胞种类生物油墨打印喷头细胞存活率参考文献
酵母细胞酵母-麦芽汁溶液F181010压电打印头96%[12]
鼠成纤维细胞胎牛血清-抗生素/抗真菌溶液30μm压电喷头94.4%[14]
新生儿包皮成纤维细胞聚乙烯吡咯烷酮基混合液50μm压电喷头95%[16]
小鼠成肌细胞无内毒素低酰基结冷胶悬浮液XAAR-126压电喷墨打印头95%[37]
仓鼠卵巢细胞磷酸盐缓冲液改进的HP51626a压电打印头90%[41]
骨髓基质细胞纤维蛋白原和凝血酶悬浮液50μm压电喷头98%[43]
表2  几种细胞压电式打印情况
图5  打印细胞冷冻贮藏
图6  癌细胞组织阵列
图7  3D血管结构
图8  细胞储存丝巢阵列
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