技术与方法 |
|
|
|
|
基于DOE设计和氨基酸补加策略提高CHO细胞表达抗CD20单克隆抗体* |
孔建涛1,庄英萍1,2,郭美锦1,2,**() |
1华东理工大学 生物反应器工程国家重点实验室 上海 200237 2上海生物制造技术协同创新中心 上海 200237 |
|
Enhancement of Anti-CD20 Monoclonal Antibody Expression by CHO based on DOE and Amino Acid Supplemental Strategy |
KONG Jian-tao1,ZHUANG Ying-ping1,2,GUO Mei-jin1,2,**() |
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China 2 Shanghai Biological Manufacturing Technology Innovation Center, Shanghai 200237, China |
[1] |
Werner R G, Noe W, Kopp K, et al. Appropriate mammalian expression systems for biopharmaceuticals. Arzneimittel-Forschung, 1998,48(8):870-880.
pmid: 9748718
|
[2] |
Raju T S. Terminal sugars of Fc glycans influence antibody effector functions of IgGs. Current Opinion in Immunology, 2008,20(4):471-478.
doi: 10.1016/j.coi.2008.06.007
pmid: 18606225
|
[3] |
Huang Y M, Hu W W, Rustandi E, et al. Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment. Biotechnology Progress, 2010,26(5):1400-1410.
pmid: 20945494
|
[4] |
Bollati-Fogolin M, Forno G, Nimtz M, et al. Temperature reduction in cultures of hGM-CSF-expressing CHO cells:Effect on productivity and product quality. Biotechnology Progress, 2005,21(1):17-21.
pmid: 15903236
|
[5] |
Yoon S K, Choi S L, Song J Y, et al. Effect of culture pH on erythropoietin production by Chinese Hamster ovary cells grown in suspension at 32.5 and 37.0℃. Biotechnology and Bioengineering, 2005,89(3):345-356.
pmid: 15625678
|
[6] |
Jardon M, Garnier A. pH, pCO2, and temperature effect on R-Adenovirus production. Biotechnology Progress, 2003,19(1):202-208.
doi: 10.1021/bp025585a
pmid: 12573026
|
[7] |
Kaufmann H, Mazur X, Fussenegger M, et al. Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. Biotechnology and Bioengineering, 1999,63(5):573-582.
doi: 10.1002/(sici)1097-0290(19990605)63:5<573::aid-bit7>3.0.co;2-y
pmid: 10397813
|
[8] |
Yoon S K, Song J Y, Lee G M. Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnology and Bioengineering, 2003,82(3):289-298.
doi: 10.1002/bit.10566
pmid: 12599255
|
[9] |
Xing Z, Kenty B, Koyrakh I, et al. Optimizing amino acid composition of CHO cell culture media for a fusion protein production. Process Biochemistry, 2011,46(7):1423-1429.
|
[10] |
Kang S, Mullen J, Miranda L P, et al. Utilization of tyrosine-and histidine-containing dipeptides to enhance productivity and culture viability. Biotechnology and Bioengineering, 2012,109(9):2286-2294.
doi: 10.1002/bit.24507
pmid: 22447498
|
[11] |
Xing Z Z, Li Z J, Chow V, et al. Identifying inhibitory threshold values of repressing metabolites in CHO cell culture using multivariate analysis methods. Biotechnology Progress, 2008,24(3):675-683.
pmid: 18422365
|
[12] |
Woolley J F, Al-Rubeai M. The application of SELDI-TOF mass spectrometry to mammalian cell culture. Biotechnology Advances, 2009,27(2):177-184.
pmid: 19049820
|
[13] |
Li F, Zhou J X, Yang X, et al. Current therapeutic antibody production and process optimization. Bioprocessing Journal, 2006,5(4):16-25.
doi: 10.12665/issn.1538-8786
|
[14] |
Feeney L, Carvalhal V, Yu X C, et al. Eliminating tyrosine sequence variants in CHO cell lines producing recombinant monoclonal antibodies. Biotechnology and Bioengineering, 2013,110(4):1087-1097.
doi: 10.1002/bit.24759
|
[15] |
庄少颖, 史劲松. 重组抗CD20单克隆抗体细胞培养工艺优化. 名医, 2020,12:349-352.
|
|
Zhuang S Y, Shi J S. The optimization of cell culture process for recombinant anti-CD20 monoclonal antibody. Renowned Doctor, 2020,12:349-352.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|