China Biotechnology

Figure/Table detail

Intelligent Production Process for PAT Controlled PRV Vaccine Based on Online Raman and Online Live Cell Detection

Nan WANG, Rui MIN, Xiaoping YI, Dahe ZHANG

China Biotechnology, 2024, 44(6): 53-67. DOI: 10.13523/j.cb.2312003

营养物质	比生长速率	营养物质	比生长速率
谷氨酰胺	+0.821 655	葡萄糖	+0.712 188
缬氨酸	+0.786 355	胱氨酸	+0.703 410
精氨酸	+0.777 453	脯氨酸	+0.679 886
赖氨酸	+0.770 222	天冬氨酸	+0.671 630
酪氨酸	+0.759 128	天冬酰胺	+0.607 778
组氨酸	+0.754 186	谷氨酸	+0.605 346
亮氨酸	+0.749 193	肌肽	+0.596 802
异亮氨酸	+0.740 944	甲硫氨酸	+0.583 191
苏氨酸	+0.736 197	色氨酸	+0.549 716
苯丙氨酸	+0.726 865	甘氨酸	-0.427 370
丝氨酸	+0.722 251	丙氨酸	-0.589 372

Table 1 Correlation coefficient table of multivariate data analysis to determine key nutrients

Other figure/table from this article

Fig.1 Figure of feeding time calculation formula
Fig.2 Related parameters of BHK-21 cells cultured at different initial glucose concentrations in the first 12 h of culture Control:6.0 g/L glc;^* P<0.05,^** P<0.01,^*** P<0.001,^**** P< 0.000 1
Fig.3 Related parameters of BHK-21 cells cultured at different supplemental concentrations of galactose in the first 12 h of culture Control 1: 1.5 g/L glc+0 gal; Control 2: 6.0 g/L glc+0 gal; ^* P<0.05,^** P<0.01,^*** P<0.001
Fig. 4 Related parameters of BHK-21 cells cultured at different initial glutamine concentrations in the first 12 h of culture Control:6.0 g/L glc+0 gal +7.0 mmol/L gln; ^* P<0.05,^** P<0.01,^*** P<0.001,^**** P<0.000 1
Fig.5 Heat map of multivariate data analysis to determine key nutrient
Table 2 Experimental design table for optimization of concentration multiple and addition amount of BHK-21 cells supplemented medium
Fig.6 Optimization of concentration multiple and addition amount of BHK-21 cells’ supplemented medium: Response surface results of 36 h cell density (A) & 36 h specific growth rate (B)
Fig.7 Related parameters of BHK-21 cells shake flask fed-batch culture in low glucose and glutamine concentration Control:6.0 g/L glc+0 gal+7.0 mmol/L gln;^* P<0.05,^** P< 0.01,^**** P<0.000 1
Fig.8 Relative maximum virus titer of PRV produced by BHK-21 cells shake flask fed-batch culture in low glucose and glutamine concentrations Control:6.0 g/L glc+0 gal+7.0 mmol/L gln;^**** P<0.000 1
Fig.9 Online parameter changes of BHK-21 cells reactor manual fed-batch culture in low glucose and glutamine concentration conditions A:No feeding B:24 h feeding 1.5 times the initial medium, 5% of the total volume;The optimal initial glucose and glutamine concentrations were 1.5 g/L and 2 mmol/L, respectively and the supplemented concentration of galactose was 6 mmol/L. During the fed-batch culture, glucose and glutamine concentrations were controlled at about 0.5 g/L and 1.5 mmol/L, respectively
Fig.10 A: High glucose control B: Reactor manual feeding
Fig.11 Cell metabolism parameters of PRV produced by BHK-21 cells under different culture conditions
Fig.12 On-line Cap and virus titers of PRV produced by BHK-21 cells under online Raman detection combined with PAT feedback control and highest relative virus titer of PRV under different culture conditions ^*** P<0.001,^**** P<0.000 1
Fig.13 Off-line/on-line detection parameters of PRV produced by BHK-21 cells under different culture conditions A: Glucose concentration curve B: Glutamine concentration curve

模态框（Modal）标题

Figure/Table detail