Abstract The objective of this research is to perfect the approach for synthesizing bataine in plants in order to improve the resistance of drought and salt by transferred CMO and BADH genes into the target plants. A bivalent-expression vector, pC35SC35SB1303 were reconstructed based on PC1303 plasmid with CMO and BADH driven by 35S promoter and introduced into Agrobacterium tumefaciens strain LBA4404 by freezethaw method. Transgenic tobacco plants recovered from leaf discs applying Agrobacteriummediated gene were detected by PCR analysis and Northern blotting. The results showed that the foreign gene was consistent with the recipient plants' genome and expresses normally. The analysis of the content of bataine in transgenic plants and blank plants showed that it was higher in the bivalent-expression vector transgenic plants than those transferred with single gene BADH or CMO and blank plants.
