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Differential Expression of 14-3-3 protein ε、S100A9 ,in Cancerization of Human Esophageal Epithelial Cells |
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Abstract ABSTRACT:Objective:To search for the biomarkers for differential diagnosis between human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells. Methods:Laser capture microdissection was used to separate human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells, Proteome alterations were observed using two dimensional polyacrylamide gel electrophoresis(2-DE) technique and electrospray ionisation tandem mass spectrometry.Westernbloting was used to detect the different protein expressions in ESCC and esophageal epithelial cells, Results : The 2-DE patterns of human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells were established. 14-3-3 protein ε、S100A9 were identified differentially expressed between ESCC and esophageal epithelial cells. Conclusion :Laser capture microdissection is a key technique in proteomic study which can effectively resolve the problem of irrelevant tissues.14-3-3 protein ε、S100A9 may serve as abiomarker for differential diagnosis of human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells.
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Received: 23 October 2007
Published: 25 February 2008
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