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Optimization of fermentation medium with lactose as a inducer for high molecular weight recombinant spider silk protein |
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Abstract The fermentation culture media for pNSR32/BL21(DE3) was investigated with shake culture method.Based on M9 medium and for the induction of lactose ,so as to determine the optimal concentration of ingredients of medium. The optimized culture medium was obtained by orthogonal and individual factor design. Optimized prescription of culture medium was investigted for the fermentation to produce the high molecular weight recombinant spider silk proteins. The optimum culture medium consisting of 0.3% glycerol,3% yeast,0.75% tryptone, 0.05%(NH4)2SO4 and little inorganic salt that was found to benefit for the growth and expression of recombinant spider silk proteins ,and to make the production level attain at 24.0%.
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Received: 27 August 2008
Published: 20 April 2009
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