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| Preparation and identification of the Monoclonal Antibody against Clenbuterol |
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Abstract The clenbuterol was conjugated with BSA by diazotization at a ratio of 17 to form CL-BSA complex. The conjugated molecule was injected into the Balb/c mice for hybridoma preparation. The results showed that the synthesized antigen could immune the mice successfully. The frequency of fusion and the positive cloning could reach 85.26% and 15.23%, respectively. Through four rounds of cloning, one optimal clone named 4H5 was obtained. The result of IELISA demonstrated that the 4H5 culture supernament was high specific activitie against CL and could reach to 1.265±0.060 (OD450nm), comparing to the ascites against BSA (OD450nm 0.060±0.006). The titer of ascites generated from 4H5 injection could reach to 3.2×106 with low affinity toward BSA, and the subtype is IgG1 with light chain κ.
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Received: 21 March 2008
Published: 01 January 1900
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