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| cyclohydrolase/Phosphoribosyl-ATP pyrophosphatase In Histidine Biosynthesis Pathway From Shigella flexneri 2a |
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Abstract The metabolic pathway of histidine biosynthesis is significant for its various gene organizations and protein structures. HisI/E gene, that can express a bifunctional phosphoribosyl-AMP-cyclohydrolase/phosphoribosyl-ATP-pyrophosphatase protein, which could catalyze the second and the third step in the histidine biosynthetic pathway, has been successfully cloned by using Shigella flexneri 2α(strain 301) genomic DNA as PCR temple. The protein was soluble expressed in pET22b vector, and purified by Ni-NTA cartridge and Superdex75 molecular filter. Then the soluble highly purified protein was characterized by preliminary crystal analysis, including dynamic light scattering, Analytical Ultracentrifugation and initially screened with Crystal Screens I and II and Index Screen (Hampton Research). The buffer of 50mmol/L Tris–HCl, pH 10.0, 300mmol/L NaCl, 0.05mmol/L ZnCl2 is the best solution for both the maintenance of oligomerization state of the protein and crystallization.
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Received: 06 November 2008
Published: 27 April 2009
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