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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (04): 78-82    DOI:
    
Chemiluminescent Detection System for Analysis the Inhibition of HBV Replication with Drugs in Different Targets
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Abstract  Objective:To stablish of chemiluminescent Southern blot detection system for examining HBV DNA replication intermediates in HepG2.2.15,and analyse the inhibition of HBV replication with three kind of drugs with different targets.Methods:The HBV DNA replication intermediates were extracted and analyzed by Southern blot with HBV probe,which(pTHBV1047) was labelling with digoxigenin.The results of the hybridization were detected by chemiluminescent,and the condition of hybridization was optimized.After treated with lamivudine,Bay41-4109, α-Galcer in different concentration,the HBV DNA from the HepG2215 cells were detected with the system.Results:the sensitivity of the system was 1pg of pTHBV1047,and HBV specific positive signals was detected with the DNA from HepG2.2.15.The three kinds of drugs can inhibit the HBV replication obviously with chemiluminescent Southern blot detection system,the IC50 were 1.53μmol/L,0.41μmol/L,0.01μmol/L. Conclusion:The HBV replication intermediates from the cell of HepG2.2.15 can reflect the antiviral effect accurately with different targets drugs and this mothod would be used in the study of Chinesemidicine.

Received: 15 January 2009      Published: 27 April 2009
Cite this article:

YI Hua-Rui- Yuan-Wei-Cheng- Chen-Wen-Jin- Su-Wei- Zhang-Feng- Liu-Zhen-Yang- Kong-Xiang-Beng. Chemiluminescent Detection System for Analysis the Inhibition of HBV Replication with Drugs in Different Targets. China Biotechnology, 2009, 29(04): 78-82.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I04/78

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