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| Reconstitution of Non-ribosomal Peptide Synthetases Catalytic Module and Peptide Synthesis |
WENG Yang-Jing,WU Jie-Qun**( ) |
| Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310000, China |
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Abstract Based on the principle of modular linear synthesis of peptides by non-ribosomal peptide synthetases (NRPS), it has become a research hotspot to engineer and recombine its catalytic module and design the biosynthetic pathway of peptides to obtain the target peptides. However, heterozygous NRPS has many problems. For example, the catalytic module cannot load the target amino acids or the synthesis efficiency of peptides is significantly reduced, which limits its application. In recent years, great breakthroughs have been made in the research of substrate selectivity of NRPS adenylation domain (A domain) and condensation domain (C domain), docking domain (DD) between NRPS subunits and linker between modules. This review introduces the research progress of NRPS catalytic module reconsitution from the two aspects of substrate selectivity in C domain and catalytic unit substitution with different fusion boundaries, and summarizes the advantages and limitations of each substitution scheme.
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Received: 08 October 2022
Published: 31 March 2023
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Corresponding Authors:
**Jie-Qun WU
E-mail: jiequnwu@zjut.edu.cn
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