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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2022, Vol. 42 Issue (12): 111-119    DOI: 10.13523/j.cb.2206061
    
Effects of Deep Eutectic Solvents on Enzyme
ZHAO Nan-shan1,2,ZHAO Ya-xin1,ZHANG Hai-hua1,YANG Dong-feng1,2,LIANG Zong-suo1,2,GUO Jian-jun1,2,**()
1 College of Life Sciences and Medicine, Zhejiang Sci-tech University, Hangzhou 310018, China
2 Zhejiang Sci-tech University Shaoxing Academy of Biomedicine, Shaoxing 312090, China
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Abstract  

Deep eutectic solvent is a new type of green solvent, which is composed of hydrogen bond acceptor and hydrogen bond donor with a certain stoichiometric ratio. It has low preparation cost and is friendly to the environment. It can be used as an alternative solvent for ordinary organic solvents and ionic liquids.As a biocatalyst, enzyme has mild reaction conditions, high substrate specificity, and high catalytic efficiency and reaction speed. Enzymatic reaction usually occurs in aqueous solution, but recently it has been found that it can also be carried out effectively in deep eutectic solvents. In order to better understand the effects of deep eutectic solvents on enzyme, the mechanism of the interaction between enzyme and deep eutectic solvents and the latest progress in application are systematically discussed. At the same time, the advantages and disadvantages of deep eutectic solvents in enzymatic reaction are evaluated. Finally, the future development prospects are discussed.

Key words： Deep eutectic solvents      Enzyme      Catalysis     
Received: 30 June 2022      Published: 05 January 2023
ZTFLH:  Q814  
Corresponding Authors: Jian-jun GUO     E-mail: biojjguo@zstu.edu.cn
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Nan-shan ZHAO
Ya-xin ZHAO
Hai-hua ZHANG
Dong-feng YANG
Zong-suo LIANG
Jian-jun GUO
Cite this article:

ZHAO Nan-shan,ZHAO Ya-xin,ZHANG Hai-hua,YANG Dong-feng,LIANG Zong-suo,GUO Jian-jun. Effects of Deep Eutectic Solvents on Enzyme. China Biotechnology, 2022, 42(12): 111-119.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2206061     OR     https://manu60.magtech.com.cn/biotech/Y2022/V42/I12/111

Fig.1 Common hydrogen bond donor and hydrogen bond receptor structures Hydrogen bond receptor: (a) Choline chloride (b) Glycine betaine (c) Sucrose; Hydrogen bond donor: (d) Urea (e) Ethylene glycol (f) Glycerin (g) Methyl urea (h) Lactic acid (i) Butylene glycol (j) Xylitol
种类 示例 主要应用领域
金属盐+季铵盐 ChCl-ZnCl2 有毒气体去除[10]
水合金属盐+季铵盐 ChCl-CrCl3·6H2O 大规模工业过程[11]
氢键供体+季铵盐 ChCl-Urea 天然产物提取[12]、金属电沉积[13-14]、制备生物柴油[15]
氢键供体+金属水合盐 MgCl2·6H2O-(CH2OH)2 合成纳米结构[16]
Table 1 Classification and application of deep eutectic solvents
原材料 低共熔溶剂 配比 辅助手段 目标产物 产量 参考文献
洋蓍草 氯化胆碱∶乳酸 1∶2 超声 总酚 (35.44 ± 2.12)mg/g [32]
爪哇龙船花 氯化胆碱∶1,2-丙二醇 1∶1 超声 阿魏酸 (12.937 ± 0.169)mg/g [33]
黄芪 氯化胆碱∶尿素 1∶2 加热 没食子酸 (1.89 ± 0.05)μg/mg [34]
鞣花酸 (12.76 ± 0.13)μg/mg
可水解单宁 (181.26 ± 0.159)μg/mg
黑胡萝卜 氯化胆碱∶柠檬酸 - 超声 花青素-3-O-糖苷 (16.855 ± 0.09)mg/g [35]
粟细糠 甜菜碱∶甘油 1∶2 超声 总酚 (7.80 ± 0.09)mg/g [36]
甜叶菊 1,2-丙二醇∶甘油∶水 8∶1∶1 超声 甜菊苷 3.48 mg/mL [37]
复方甘草片 1,4-丁二醇∶乙酰丙酸 1∶3 超声 甘草苷 5.6 mg/g [38]
异甘草苷 3.17 mg/g
甘草素 1.27 mg/g
甘草酸 74.62 mg/g
异甘草素 1.34 mg/g
文冠果 四丙基溴化铵∶乳酸 1∶2 加热 皂苷 (72.11 ± 0.61)mg/g [39]
鸡矢藤 氯化胆碱∶乙二醇 1∶2 超声 总黄酮 27.04 mg/g [26]
显齿蛇葡萄叶 氯化胆碱∶葡萄糖 4∶1 超声 黄酮 83.93% [27]
云杉叶 氯化胆碱∶二羧酸 1∶1 - 黄酮 217.56 μg/mL [12]
Table 2 Extraction of plant active ingredients with deep eutectic solvents
Fig.2 Production process of deep eutectic solvents and lignofibrous biomass
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[3] Shuang JIN,Yun-song YANG,Jin-hua LIANG,Xiao-rui YANG,Xiao-tong LI,Jian-liang ZHU. Study on the Performance of Oxidative Diesel Desulfurization Catalyzed by Bioenzyme/γ-Al2O3 Spheres[J]. China Biotechnology, 2022, 42(10): 21-30.
[4] MIAO Yi-nan,LI Jing-zhi,WANG Shuai,LI Chun,WANG Ying. Research Progress of Key Enzymes in Terpene Biosynthesis[J]. China Biotechnology, 2021, 41(6): 60-70.
[5] ZHANG Lei,TANG Yong-kai,LI Hong-xia,LI Jian-lin,XU Yu-xin,LI Ying-bin,YU Ju-hua. Advances in Promoting Solubility of Prokaryotic Expressed Proteins[J]. China Biotechnology, 2021, 41(2/3): 138-149.
[6] YANG Yun-song,LIANG Jin-hua,YANG Xiao-rui,MA Yi-ming,JIN Shuang,SUN Yao-yao,ZHU Jian-liang. Research Progress in Oxidative Desulfurization of Diesel Oil Catalyzed by Enzymes[J]. China Biotechnology, 2021, 41(10): 109-115.
[7] WANG Guang-lu, WANG Meng-yuan, ZHOU Yi-fei, MA Ke, ZHANG Fan, YANG Xue-peng. Research Progress in Pyrrologuinoline Quinone Biosynthesis[J]. China Biotechnology, 2021, 41(1): 103-113.
[8] WU Hong-xuan, YANG Jin-hua, SHEN Pei-jie, LI Qing-chen, HUANG Jian-zhong, QI Feng. Study on the Production of Indole-3-acetic Acid Using E. coli Cell Factory[J]. China Biotechnology, 2021, 41(1): 12-19.
[9] DONG Lu,ZHANG Ji-fu,ZHANG Yun,HU Yun-feng. Immobilization of Extracellaluar Proteases of Bacillus sp. DL-2 Using Epoxy Resin to Asymmetrically Hydrolyze (±)-1-Phenylethyl Acetate[J]. China Biotechnology, 2020, 40(4): 49-58.
[10] LE Yi-lin,FU Yu,NI Li,SUN Jian-zhong. Expression and Characterization of a Thermostable Pyruvate Ferredoxin Oxidoreductase from the Hyperthermophile Thermotoga neapolitana and Its Application in Acetyl-CoA Production[J]. China Biotechnology, 2020, 40(3): 72-78.
[11] CHEN Xin-yi,LIU Hu,DAI Da-zhang,LI Chun. Strategies to Improve Crystallizability of Glycosylated Enzyme[J]. China Biotechnology, 2020, 40(3): 154-162.
[12] LI Bing-juan,LIU Jin-ding,LIAO Yi-fang,HAN Wen-ying,LIU Ke,HOU Chen-lu,ZHANG Lei. Advances in Protein Engineering of the Old Yellow Enzyme OYE Family[J]. China Biotechnology, 2020, 40(3): 163-169.
[13] LI Zhi-gang,CHEN Bao-feng,ZHANG Zhong-hua,CHANG Jing-ling. The Physiological Mechanism for Enhanced Cyclic Adenosine Monophosphate Biosynthesis by Auxiliary Energy Substance[J]. China Biotechnology, 2020, 40(1-2): 102-108.
[14] HU Yan-hong,GONG Xue-mei,Ding Liu-liu,GAO Song,LI Ting-ting. Highly Efficient Expression and Purification of Ketoreductase CgKR2 Using Brevibacillus choshinensis SP3[J]. China Biotechnology, 2019, 39(8): 59-65.
[15] Heng ZHU,Hai-jiao LIN,Ji-fu ZHANG,Yun ZHANG,Ai-jun SUN,Yun-feng HU. Covalent Immobilization of Marine Candida Rugosa Lipase Using Amino Carrier[J]. China Biotechnology, 2019, 39(7): 71-78.
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