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Protective Effects of Copper Nanoparticles Against the Neurovascular Unit after Ischemic Stroke |
HUANG Ji-an,LI Wan-meng,LIU Wei,QI Zi-tong,ZHAO Liang() |
School of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China |
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Abstract Objective: To study the protective effect of copper nanoparticles (Cu-nps) on the neurovascular units(NVU) after ischemic stroke. Methods: Cu-nps was synthesized by heating and stirring method. A model of rat transient middle cerebral artery occlusion/ reperfusion (tMCAO) was established in vivo. The experiment was divided into three groups, including normal group (Sham), model group (tMCAO), and dosing group (Cu-nps). Brain infarction area, neural apoptosis, blood-brain barrier (BBB) integrity, and related protein expression were detected in each group. Results: Cu-nps were successfully prepared and present in the form of Cu2+ with a uniform particle size around 80 nm and good biocompatibility. Cu-nps target damaged neurons in cerebral ischemia, improve neuronal cell viability, reduce reactive oxygen species (ROS), reduce neuronal apoptosis and cerebral infarct area, and reduce EB dye leakage and inflammatory cytokine expression. Conclusion: Cu-nps reduce cerebral ischemia-reperfusion injury, protect BBB integrity, reduce oxidative stress, protect NVU function and therefore reduce glial cell activation.
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Received: 13 July 2022
Published: 05 January 2023
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Corresponding Authors:
Liang ZHAO
E-mail: liangzhao79@163.com
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[1] |
Campbell B C V, de Silva D A, Macleod M R, et al. Ischaemic stroke. Nature Reviews Disease Primers, 2019, 5(1): 70.
doi: 10.1038/s41572-019-0118-8
pmid: 31601801
|
|
|
[2] |
Shen Z, Zheng Y R, Wu J Y, et al. PARK2-dependent mitophagy induced by acidic postconditioning protects against focal cerebral ischemia and extends the reperfusion window. Autophagy, 2017, 13(3): 473-485.
doi: 10.1080/15548627.2016.1274596
pmid: 28103118
|
|
|
[3] |
Iadecola C. The neurovascular unit coming of age: a journey through neurovascular coupling in health and disease. Neuron, 2017, 96(1): 17-42.
doi: S0896-6273(17)30652-9
pmid: 28957666
|
|
|
[4] |
Ni M M, You Y P, Chen J Y, et al. Copper in depressive disorder: a systematic review and meta-analysis of observational studies. Psychiatry Research, 2018, 267: 506-515.
doi: S0165-1781(17)31913-3
pmid: 29980131
|
|
|
[5] |
Lanza V, Milardi D, di Natale G, et al. Repurposing of copper(II)-chelating drugs for the treatment of neurodegenerative diseases. Current Medicinal Chemistry, 2018, 25(4): 525-539.
doi: 10.2174/0929867324666170518094404
|
|
|
[6] |
Southon A, Szostak K, Acevedo K M, et al. CuII(atsm) inhibits ferroptosis: implications for treatment of neurodegenerative disease. British Journal of Pharmacology, 2020, 177(3): 656-667.
doi: 10.1111/bph.14881
pmid: 31655003
|
|
|
[7] |
Pedone D, Moglianetti M, de Luca E, et al. Platinum nanoparticles in nanobiomedicine. Chemical Society Reviews, 2017, 46(16): 4951-4975.
doi: 10.1039/c7cs00152e
pmid: 28696452
|
|
|
[8] |
Tian X, Fan T J, Zhao W T, et al. Recent advances in the development of nanomedicines for the treatment of ischemic stroke. Bioactive Materials, 2021, 6(9): 2854-2869.
doi: 10.1016/j.bioactmat.2021.01.023
pmid: 33718667
|
|
|
[9] |
Zhao L P, Ji C, Lu P H, et al. Oxygen glucose deprivation (OGD)/re-oxygenation-induced in vitro neuronal cell death involves mitochondrial cyclophilin-D/P 53 signaling axis. Neurochemical Research, 2013, 38(4): 705-713.
doi: 10.1007/s11064-013-0968-5
|
|
|
[10] |
Wu L, Zhang K, Sun L P, et al. Laminin degradation by matrix metalloproteinase 9 promotes ketamine-induced neuronal apoptosis in the early developing rat retina. CNS Neuroscience & Therapeutics, 2020, 26(10): 1058-1068.
|
|
|
[11] |
He R Y, Jiang Y B, Shi Y J, et al. Curcumin-laden exosomes target ischemic brain tissue and alleviate cerebral ischemia-reperfusion injury by inhibiting ROS-mediated mitochondrial apoptosis. Materials Science and Engineering C, Materials for Biological Applications, 2020, 117: 111314.
doi: 10.1016/j.msec.2020.111314
|
|
|
[12] |
Tiwari Y V, Lu J, Shen Q, et al. Magnetic resonance imaging of blood-brain barrier permeability in ischemic stroke using diffusion-weighted arterial spin labeling in rats. Journal of Cerebral Blood Flow and Metabolism, 2017, 37(8): 2706-2715.
doi: 10.1177/0271678X16673385
pmid: 27742887
|
|
|
[13] |
Ye X C, Hao Q, Ma W J, et al. Dectin-1/Syk signaling triggers neuroinflammation after ischemic stroke in mice. Journal of Neuroinflammation, 2020, 17(1): 17.
doi: 10.1186/s12974-019-1693-z
|
|
|
[14] |
Liu W L, Sood R, Chen Q C, et al. Normobaric hyperoxia inhibits NADPH oxidase-mediated matrix metalloproteinase-9 induction in cerebral microvessels in experimental stroke. Journal of Neurochemistry, 2008, 107(5): 1196-1205.
doi: 10.1111/j.1471-4159.2008.05664.x
pmid: 18786175
|
|
|
[15] |
Meng F H, Shi W, Sun Y N, et al. Nonenzymatic biosensor based on CuxO nanoparticles deposited on polypyrrole nanowires for improving detectionrange. Biosensors and Bioelectronics, 2013, 42: 141-147.
doi: 10.1016/j.bios.2012.10.051
|
|
|
[16] |
成小蔓, 王永红, 周远大, 等. 雌二醇对去势大鼠局灶性脑缺血再灌注后Ang-1mRNA表达的影响. 中国生物工程杂志, 2008, 28(S1): 16-20.
|
|
|
[16] |
Cheng X M, Wang Y H, Zhou Y D, et al. The effects of estradiol on angiopoietin-1mRNA expression in a castrated rat with focal cerebral ischemia after reperfusion. China Biotechnology, 2008, 28(S1): 16-20.
|
|
|
[17] |
Cai W, Zhang K, Li P Y, et al. Dysfunction of the neurovascular unit in ischemic stroke and neurodegenerative diseases: an aging effect. Ageing Research Reviews, 2017, 34: 77-87.
doi: S1568-1637(16)30117-9
pmid: 27697546
|
|
|
[18] |
Roberts B R, Lim N K, McAllum E J, et al. Oral treatment with Cu(II)(atsm) increases mutant SOD1 in vivo but protects motor neurons and improves the phenotype of a transgenic mouse model of amyotrophic lateral sclerosis. The Journal of Neuroscience, 2014, 34(23): 8021-8031.
doi: 10.1523/JNEUROSCI.4196-13.2014
|
|
|
[19] |
Furtado D, Björnmalm M, Ayton S, et al. Overcoming the blood-brain barrier: the role of nanomaterials in treating neurological diseases. Advanced Materials, 2018, 30(46): e1801362.
|
|
|
[20] |
Zhang K, Tu M J, Gao W, et al. Hollow Prussian blue nanozymes drive neuroprotection against ischemic stroke via attenuating oxidative stress, counteracting inflammation, and suppressing cell apoptosis. Nano Letters, 2019, 19(5): 2812-2823.
doi: 10.1021/acs.nanolett.8b04729
pmid: 30908916
|
|
|
[21] |
Xie J B, Shen Z Y, Anraku Y, et al. Nanomaterial-based blood-brain-barrier (BBB) crossing strategies. Biomaterials, 2019, 224: 119491.
doi: 10.1016/j.biomaterials.2019.119491
|
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