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Serum Metabolomics of Ophiopogon japonicus Extract Against Type 2 Diabetes in Mice |
ZHANG Jie1,2, LIN Bing-feng2, XU Ping-cui2, WANG Na-ni2, CHEN Yu1,**() |
1 Hangzhou Vocational & Technical College, Hangzhou 310018, China 2 Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China |
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Abstract Objective: To study the protective mechanism of Ophiopogon japonicus extract on Type 2 diabetes mellitus (T2DM) based on metabolomics. Methods: The T2DM mouse model was established by intraperitoneal injection of streptozotocin. Mice were orally administrated with the aqueous extract of Ophiopogon japonicu for 4 weeks. The diabetic phenotypes in serum were measured. Changes in serum metabolites were determined by ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS). Results: Compared with the control group, the fasting glucose, total cholesterol, triglyceride, and low-density lipoprotein cholesterol were increased in the model group, while the high-density lipoprotein cholesterol decreased significantly, and the above indexes were reversed after treatment of Ophiopogon japonicus extract. The metabonomic results showed that 43 differential metabolites were found between the control group and the model group. These metabolites were enriched in 18 pathways. Ophiopogon japonicus extract significantly decreased the content of glyceric acid, malonic semialdehyde, and 4-hydroxyphenylpyruvic acid in T2DM mice. Ophiopogon japonicus extract could regulate 7 pathways, such as ubiquinone and other terpenoid-quinone biosynthesis. Conclusion: The hypoglycemic and lipid-lowering effect of Ophiopogon on T2DM may be related to ubiquinone and other terpenoid-quinone biosynthesis.
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Received: 03 September 2022
Published: 07 December 2022
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