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Overexpression of TPP from Larix gmelinii Enhanced Salt Tolerance of the Transgenic Arabidopsis thaliana |
ZHANG Yan-xia1,ZHANG Xu-ting1,JIA Yong-hong2,LEI Feng-yan3,WANG Jing1,WANG Rui-gang1,4,LI Guo-jing1,**() |
1. Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China 2. Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109, China 3. Agriculture and Animal Husbandry Bureau of Ongniud Banner of Tongliao City, Tongliao 028000, China 4. Inner Mongolia Enterprise Key Laboratory of Tree Breeding, Mengshu Ecological Construction Group Co.,Ltd., Hohhot 011517, China |
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Abstract Larix gmelinii is a very important coniferous tree species for afforestation. It has the characteristics of rapid growth in early stage, strong stress resistance and good ecological benefits. Trehalose participates in the regulation of drought, cold, salt damage and other stresses. Trehalose-6-phosphate phosphatase (TPP) is an important enzyme in the trehalose synthesis pathway. In this study, the full-length sequence of LgTPPI.1 was screened from the transcriptome of L. gmelinii under stress and its coding sequence(CDS) was cloned. The recombinant vector was constructed and homozygous lines of transgenic Arabidopsis thaliana overexpressing LgTPPI.1 were obtained. The results showed that the full-length CDS of LgTPPI.1 was 1 236 bp, encoding 411 amino acids; the expression level of LgTPPI.1 was lower in roots and stems, but higher in needles. Under salt treatment, the LgTPPI.1 overexpression lines conferred stronger tolerance than the wild type A. thaliana, with elevated trehalose and proline content; increased superoxide dismutase(SOD) and catalase(CAT) activity and up-regulated expresssion of the stress-responsive marker genes. These results indicated that gymnosperm utilized similar trehalose pathway as angiosperm to telerate abiotic stress. This study provided a theoretical basis for further analysis of the function of trehalose synthetic genes and the response mechanism of conifers under stress.
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Received: 28 March 2022
Published: 07 September 2022
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Corresponding Authors:
Guo-jing LI
E-mail: liguojing@imau.edu.cn
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