|
|
|
| Osteogenic Properties of Dental-derived Stem Cell Composite with Grooved Porous Hydroxyapatite Scaffolds |
LAI Shuang1,2,LIU Chang1,LIU Chun-hui2,LIU Cong2,REN Xiao-hua1,2,**( ),MU Yan-dong1,2,**() |
1. Affiliated Hospital of University of Electronic Science and Technology of China,Sichuan Provincial People’s Hospital, Chengdu 610072, China
2. University of Electronic Science and Technology of China, Medical School,Chengdu 610072, China |
|
|
|
Abstract Objective: To investigate the osteogenic properties of dental-derived stem cell composite with grooved porous hydroxyapatite (HAG) scaffolds to provide a new approach for bone defect repair. Methods: Periodontal ligament stem cells (PDLSCs) and dental pulp stem cells (DPSCs) were separated from healthy adults’ third molars and seeded on HAG scaffolds for multi-directional differentiation identification and alkaline phosphatase (ALP) activity detection; the cell proliferation ability was detected by CCK-8; reverse transcription polymerase chain reaction (qRT-PCR) was used to detect bone morphogenetic protein 2 (BMP-2), osteocalcin (OCN) and osteopontin (OPN).In vivo study, the HAG scaffolds loaded with two kinds of cells were transplanted into the back subcutaneous tissue of nude mice, and the tissue engineered bones were taken after 8 weeks. After tissue sections, hematoxylin and eosin (HE) staining was used to observe the formation of new bones. Western blot was used to observe expressions of osteogenesis-related proteins such as ALP and OCN. Results: In the in vitro study, the cell proliferation ability, ALP activity, and the expressions of osteogenesis-related genes ALP, BMP2, and OCN in the DPSCs combined with HAG scaffold group were higher than those in the PDLSCs combined with HAG scaffold group. In vivo study, HE staining showed that the PDLSCs combined with HAG scaffold group and the DPSCs combined with HAG scaffold group had more cell growth areas, fibrocyte proliferation and bone matrix formation than the blank HAG scaffold group, and the DPSCs combined with HAG scaffold group had more bone matrix area. The expression of bone-related proteins in the PDLSCs combined with HAG scaffold group and the DPSCs combined with HAG scaffold group was higher than that in the blank HAG group, and the expression of ALP protein in the DPSCs combined with HAG scaffold group was significantly higher than that in the PDLSCs combined with HAG scaffold group. Conclusion: PDLSC and DPSC composites with HAG scaffolds showed good osteogenic properties in vitro and in vivo, and the osteogenic properties of DPSC composite with HAG scaffolds were even better.
|
|
Received: 28 March 2022
Published: 07 September 2022
|
|
|
|
Corresponding Authors:
Xiao-hua REN,Yan-dong MU
E-mail: 1072718910@qq.com;995448060@qq.com
|
|
|
| [1] |
Roseti L, Parisi V, Petretta M, et al. Scaffolds for Bone Tissue Engineering: state of the art and new perspectives. Materials Science and Engineering: C, 2017, 78: 1246-1262.
doi: 10.1016/j.msec.2017.05.017
|
|
|
| [2] |
Qu H W, Fu H Y, Han Z Y, et al. Biomaterials for bone tissue engineering scaffolds: a review. RSC Advances, 2019, 9(45): 26252-26262.
doi: 10.1039/C9RA05214C
|
|
|
| [3] |
Koons G L, Diba M N, Mikos A G. Materials design for bone-tissue engineering. Nature Reviews Materials, 2020, 5(8): 584-603.
doi: 10.1038/s41578-020-0204-2
|
|
|
| [4] |
Shang F Q, Yu Y, Liu S Y, et al. Advancing application of mesenchymal stem cell-based bone tissue regeneration. Bioactive Materials, 2021, 6(3): 666-683.
doi: 10.1016/j.bioactmat.2020.08.014
|
|
|
| [5] |
Song N, Scholtemeijer M, Shah K. Mesenchymal stem cell immunomodulation: mechanisms and therapeutic potential. Trends in Pharmacological Sciences, 2020, 41(9): 653-664.
doi: S0165-6147(20)30145-0
pmid: 32709406
|
|
|
| [6] |
Wang H H, Zhong Q, Yang T S, et al. Comparative characterization of SHED and DPSCs during extended cultivation in vitro. Molecular Medicine Reports, 2018, 17(5): 6551-6559.
|
|
|
| [7] |
Ansari S, Seagroves J T, Chen C, et al. Dental and orofacial mesenchymal stem cells in craniofacial regeneration: the prosthodontist’s point of view. The Journal of Prosthetic Dentistry, 2017, 118(4): 455-461.
doi: 10.1016/j.prosdent.2016.11.021
|
|
|
| [8] |
Kim B C, Bae H, Kwon I K, et al. Osteoblastic/cementoblastic and neural differentiation of dental stem cells and their applications to tissue engineering and regenerative medicine. Tissue Engineering Part B, Reviews, 2012, 18(3): 235-244.
doi: 10.1089/ten.teb.2011.0642
|
|
|
| [9] |
Volponi A A, Pang Y, Sharpe P T. Stem cell-based biological tooth repair and regeneration. Trends in Cell Biology, 2010, 20(12): 715-722.
doi: 10.1016/j.tcb.2010.09.012
|
|
|
| [10] |
黄智蕴, 王娟, 叶青松, 等. 牙髓干细胞治疗颌面部缺损的应用潜力. 临床口腔医学杂志, 2021, 37(7): 436-439.
|
|
|
| [10] |
Huang Z Y, Wang J, Ye Q S, et al. Application potential of dental pulp stem cells in the treatment of maxillofacial defects. Journal of Clinical Stomatology, 2021, 37(7): 436-439.
|
|
|
| [11] |
赵艳, 刘佳, 秦文, 等. 不同等级应力对人炎症牙周膜干细胞分化及细胞骨架重组的研究. 临床口腔医学杂志, 2019, 35(3): 131-135.
|
|
|
| [11] |
Zhao Y, Liu J, Qin W, et al. The effects of different magnitudes of static mechanical strain on the differentiation and cytoskeletal reorganization of human periodontal ligament stem cells in an inflammatory condition in vitro. Journal of Clinical Stomatology, 2019, 35(3): 131-135.
|
|
|
| [12] |
Sequeira D B, Oliveira A R, Seabra C M, et al. Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials. Clinical Oral Investigations, 2021, 25(9): 5317-5329.
doi: 10.1007/s00784-021-03840-9
pmid: 33630165
|
|
|
| [13] |
Yamakawa D, Kawase-Koga Y, Fujii Y, et al. Effects of helioxanthin derivative-treated human dental pulp stem cells on fracture healing. International Journal of Molecular Sciences, 2020, 21(23): 9158.
doi: 10.3390/ijms21239158
|
|
|
| [14] |
Mondal B, Mondal S, Mondal A, et al. Fish scale derived hydroxyapatite scaffold for bone tissue engineering. Materials Characterization, 2016, 121: 112-124.
doi: 10.1016/j.matchar.2016.09.034
|
|
|
| [15] |
Ren X H, Tuo Q, Tian K, et al. Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro. Ceramics International, 2018, 44(17): 21656-21665.
doi: 10.1016/j.ceramint.2018.08.249
|
|
|
| [16] |
Li C L, Yang L, Ren X H, et al. Groove structure of porous hydroxyapatite scaffolds (HAS) modulates immune environment via regulating macrophages and subsequently enhances osteogenesis. JBIC Journal of Biological Inorganic Chemistry, 2019, 24(5): 733-745.
doi: 10.1007/s00775-019-01687-w
|
|
|
| [17] |
温永梅, 牟雁东, 伍佳, 等. 新型多孔羟基磷灰石陶瓷支架在骨内成骨的初步评价. 中国口腔种植学杂志, 2017, 22(1): 9-12, 28.
|
|
|
| [17] |
Wen Y M, Mu Y D, Wu J, et al. New type of porous hydroxyapatite ceramic scaffolds in bone ossification of preliminary evaluation. Chinese Journal of Oral Implantology, 2017, 22(1): 9-12, 28.
|
|
|
| [18] |
吕欣荣, 温永梅, 伍佳, 等. 微渠表面多孔羟基磷灰石支架体内异位成骨性的研究. 实用医院临床杂志, 2017, 14(3): 28-31.
|
|
|
| [18] |
Lv X R, Wen Y M, Wu J, et al. Osteogenesis of micro-grooved patterns of porous hydroxyapatite scaffolds in non-osseous tissue. Practical Journal of Clinical Medicine, 2017, 14(3): 28-31.
|
|
|
| [19] |
Al-Habib M, Huang G T J. Dental mesenchymal stem cells: dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells-isolation, characterization, and expansion for tissue engineering. Methods in Molecular Biology (Clifton, N J), 2019, 1922: 59-76.
|
|
|
| [20] |
di Cosola M, Cantore S, Balzanelli M G, et al. Dental-derived stem cells and biowaste biomaterials: What’s next in bone regenerative medicine applications. BIOCELL, 2022, 46(4): 923-929.
doi: 10.32604/biocell.2022.018409
|
|
|
| [21] |
Fang F C, Zhang K Y, Chen Z, et al. Noncoding RNAs: new insights into the odontogenic differentiation of dental tissue-derived mesenchymal stem cells. Stem Cell Research & Therapy, 2019, 10(1): 297.
|
|
|
| [22] |
Osathanon T, Manokawinchoke J, Nowwarote N, et al. Notch signaling is involved in neurogenic commitment of human periodontal ligament-derived mesenchymal stem cells. Stem Cells and Development, 2013, 22(8): 1220-1231.
doi: 10.1089/scd.2012.0430
|
|
|
| [23] |
Chen Y T, Huang H C, Li G X, et al. Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine. Stem Cell Research & Therapy, 2022, 13(1): 38.
|
|
|
| [24] |
Mahmoud E M, Sayed M, El-Kady A M, et al. In vitro and in vivo study of naturally derived alginate/hydroxyapatite bio composite scaffolds. International Journal of Biological Macromolecules, 2020, 165: 1346-1360.
doi: 10.1016/j.ijbiomac.2020.10.014
pmid: 33038401
|
|
|
| [25] |
Vasandan A B, Shankar S R, Prasad P, et al. Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament. Journal of Cellular and Molecular Medicine, 2014, 18(2): 344-354.
doi: 10.1111/jcmm.12192
pmid: 24393246
|
|
|
| [26] |
Lei M, Li K, Li B, et al. Mesenchymal stem cell characteristics of dental pulp and periodontal ligament stem cells after in vivo transplantation. Biomaterials, 2014, 35(24): 6332-6343.
doi: 10.1016/j.biomaterials.2014.04.071
|
|
|
| [27] |
Zhou L L, Liu W, Wu Y M, et al. Oral mesenchymal stem/progenitor cells: the immunomodulatory Masters. Stem Cells International, 2020, 2020: 1327405.
|
|
|
| [28] |
Wada N, Menicanin D, Shi S T, et al. Immunomodulatory properties of human periodontal ligament stem cells. Journal of Cellular Physiology, 2009, 219(3): 667-676.
doi: 10.1002/jcp.21710
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
