Research and Application Progress of Signal Peptides in <i>Escherichia coli</i> Secretion Systems

doi:10.13523/j.cb.2101018

China Biotechnology

2021, Vol. 41

Issue (5): 87-93 DOI: 10.13523/j.cb.2101018

Research and Application Progress of Signal Peptides in Escherichia coli Secretion Systems

HE Ruo-yu^1,²,LIN Fu-yu²,GAO Xiang-dong^1,^*(

),LIU Jin-yi^2,^*(

)

1 School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
2 Beijing Tri-Prime Genetic Engineering Co., Ltd, Beijing 102600, China

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Abstract

Escherichia coli has become a commonly used system for expressing recombinant proteins due to its obvious advantages. However, E. coli does not have the oxidative conditions and molecular mechanisms for the formation of disulfide bonds in the cytoplasm, and high-level expression often tends to aggregate to form inclusion bodies, which limits its use. Signal peptides to guide proteins secretion can overcome this shortcoming. The proteins with signal peptides are secreted by the Sec or/and Tat systems in E. coli. The E. coli secretion systems and the structure of signal peptides were summarized. Meanwhile, the research and application progress of six commonly used signal peptides in recent years were introduced. Finally, the problems and improvement measures in the application of signal peptides were summarized,which would provide more useful information and strategies for researchers to select signal peptides reasonably and optimize the expression of recombinant proteins.

Key words： Signal peptide Escherichia coli Secretion system

Received: 14 January 2021 Published: 01 June 2021

ZTFLH:

Q816

Corresponding Authors: Xiang-dong GAO,Jin-yi LIU E-mail: xdgao@cpu.edu.cn;liujinyi@triprime.com

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	Ruo-yu HE
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Cite this article:

HE Ruo-yu,LIN Fu-yu,GAO Xiang-dong,LIU Jin-yi. Research and Application Progress of Signal Peptides in Escherichia coli Secretion Systems. China Biotechnology, 2021, 41(5): 87-93.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2101018 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I5/87

Table 1 Six commonly used signal peptides in E. coli system


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