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| Advances in Promoting Solubility of Prokaryotic Expressed Proteins |
ZHANG Lei1,TANG Yong-kai1,2,LI Hong-xia1,2,LI Jian-lin1,2,XU Yu-xin1,LI Ying-bin1,YU Ju-hua1,2,**( ) |
1 Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu 214128, China
2 Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214128, China |
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Abstract The prokaryotic expression protein system, especially Escherichia coli expression system, is a preferred method for obtaining exogenous proteins because of its advantages of simple operation, short cycle, high expression level and relatively low cost. However, one of the major factors to limit the extensive application of E.coli expression protein system is that the exogenous proteins, when expressed in a high amount, are often present in an insoluble form. With the understanding of protein folding dynamics, enzymes and molecular chaperones involved in protein folding, scientists have made great progress in promoting solubility of heterologous proteins by changing expression conditions, modifying host cells or using fusion tags. Here, we review the related progress in order to provide reference for related research.
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Received: 11 June 2020
Published: 08 April 2021
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Corresponding Authors:
Ju-hua YU
E-mail: yujh@ffrc.cn
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