Adhesion and proliferation of bone marrow stromal cells with PLGA-ASP-PEG polymer scaffolds

China Biotechnology

技术与方法

Adhesion and proliferation of bone marrow stromal cells with PLGA-ASP-PEG polymer scaffolds

Duan Zhi-xia Zheng Qi-xin

Download:

HTML

PDF(0KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract Objectives: To investigate the effects of adhesion and proliferation of bone mesenchymal stem cells (BMSCs) in the surface of lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol PLGA-[ASP-PEG] tri-block polymer scaffolds, try to find a new biomaterial to induce seed cells in vitro for bone tissue engineering. Methods: Modified PLGA with polyethylene glycol (PEG) and asparagic acid (ASP) that has many ligands, and synthesis PLGA-[ASP-PEG] polymer material. BMSCs were cultured in PLGA-[ASP-PEG] polymer material and PLGA used as control group. Through precipitation method, MTT assay and total cellular protein detection to test the adhersion and proliferation of BMSCs. Scanning electron microscope is used to observe cells appearance. Results: BMSCs on the surface of PLGA-[ASP-PEG] polymer scaffolds are adherention to the culture flask, the number of cell's is much higher than PLGA's. The precipitation method suggest that adhesion and proliferation of BMSCs on the surface of PLGA-[ASP-PEG] is much higher then the control group. P＜0.05. MTT assay show: after culture on PLGA-[ASP-PEG] polymer scaffolds for 20 days, the absorbance A is 1.336, and PLGA as control group is 0.780. Total cellular protein can image the adhersion and proliferation of BMSCs indirectly. After 12 days culture, the total cellular protein of PLGA-[ASP-PEG] is 66.44μg/pore, and PLGA is 41.23μg/pore, that is to say PLGA-[ASP-PEG] polymer scaffolds have well biocompatibility and cells adhersion. Conclusion: PLGA-[ASP-PEG] can improve the seed cell's adhesion and proliferation, and keep cells in good condition.

Key words： proliferation osteodifferentiation bone marrow stromal cells polymer material

Received: 24 March 2006 Published: 25 December 2006

Corresponding Authors: Duan Zhi-xia

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Duan Zhi-xia Zheng Qi-xin
	Duan Zhi-xia Zheng Qi-xin
	Duan Zhi-xia Zheng Qi-xin

Cite this article:

Duan Zhi-xia Zheng Qi-xin . Adhesion and proliferation of bone marrow stromal cells with PLGA-ASP-PEG polymer scaffolds. China Biotechnology, 2006, 26(12): 86-91.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I12/86

[1]	LI Shi-rong,CHEN Yang-qin,ZHANG Chun-pan,QI Wen-jie. RS4651 Inhibits the EMT of Mouse Hepatocyte AML12 via Upregulating SMAD7[J]. China Biotechnology, 2021, 41(7): 1-9.

[2]	OUYANG Qin,LI Yan-meng,XU An-jian,ZHOU Dong-hu,LI Zhen-kun,HUANG Jian. GTF2H2 Affects the Proliferation and Migration of Hep3B Hepatocellular Carcinoma Cells by Mediating AKT Signal Pathway[J]. China Biotechnology, 2021, 41(6): 4-12.

[3]	TAO Shou-song,REN Guang-ming,YIN Rong-hua,YANG Xiao-ming,MA Wen-bing,GE Zhi-qiang. Knockdown of Deubiquitinase USP13 Inhibits the Proliferation of K562 Cells[J]. China Biotechnology, 2021, 41(5): 1-7.

[4]	LU Yu-xiang,LI Yuan,FANG Dan-dan,WANG Xue-bo,YANG Wan-peng,CHU Yuan-kui,YANG Hua. The Role and Expression Regulation of MiR-5047 in the Proliferation and Migration of Breast Cancer Cells[J]. China Biotechnology, 2021, 41(4): 9-17.

[5]	TANG Min,WAN Qun,SUN Shi-lei,HU Jing,SUN Zi-jiu,FANG Yu-ting,ZHANG Yan. The Effects of Hsa-miR-5195-3p on the Proliferation, Migration and Invasion of Human Cervical Cancer SiHa Cells[J]. China Biotechnology, 2020, 40(4): 17-24.

[6]	YANG Dan,TIAN Hai-shan,LI Xiao-kun. Research Progress of Fibroblast Growth Factor 5[J]. China Biotechnology, 2020, 40(3): 117-124.

[7]	GU Hao,GUO Xin-yu,DU Jing-jing,ZHANG Pei-wen,WANG Ding-guo,LIAO Kun,ZHANG Shun-hua,ZHU Li. The Effect of miR-186-5p on the Proliferation and Differentiation of 3T3-L1 Preadipocyte[J]. China Biotechnology, 2020, 40(3): 21-30.

[8]	HE Xiu-juan,HU Feng-zhi,LIU Qiu-li,LIU Yu-ping,ZHU Ling,ZHENG Wen-yun. CRISPR / Cas9 Gene Editing of QSOX1 in Breast Cancer Cells and Its Effect on the Proliferation and Invasion[J]. China Biotechnology, 2020, 40(11): 1-9.

[9]	Lu WANG,Li-yuan YANG,Yu-ting TANG,Yao TAO,Li LEI,Yi-pei JING,Xue-ke JIANG,Ling ZHANG. Effects of PKM2 Knockdown on Proliferation and Apoptosis of Human Leukemia Cells and Its Potential Mechanism[J]. China Biotechnology, 2019, 39(3): 13-20.

[10]	DUAN Li-mei,YANG Jin-xiao,LIU Jia-yu,ZHENG Yong-bo,WU Xiao-hou,LUO Chun-li. shPLCε Inhibits Serine/Glycine Metabolism and Proliferation of Prostate Cancer via YAP Signaling Pathway[J]. China Biotechnology, 2019, 39(11): 1-12.

[11]	Qun WAN,Meng-yao LIU,Jing XIA,Li-yao GOU,Min TANG,Shi-lei SUN,Yan ZHANG. The Effects of LncRNA SNHG3 on the Proliferation, Migration and Invasion of Human Breast Cancer MCF-7 Cells[J]. China Biotechnology, 2019, 39(1): 13-20.

[12]	Li-yao GOU,Meng-yao LIU,Jing XIA,Qun WAN,Chi-lei SUN,Min TANG,Yan ZHANG. The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells[J]. China Biotechnology, 2018, 38(5): 10-16.

[13]	Yi-man LI,Qin ZHOU. *The Effects of Herpud1* on Metanephric Mesenchymal Cells and Its Mechanism**[J]. China Biotechnology, 2018, 38(3): 9-15.

[14]	Qiong YANG,Ling-hui WANG,Hao GU,Jing-jing DU,Jin-yuan LIU,Shun-hua ZHANG,Li ZHU. The Effect of miR-196a-5p on Proliferation and Differentiation of 3T3-L1 Preadipocyte[J]. China Biotechnology, 2018, 38(11): 9-17.

[15]	FENG Yuan, TANG Yun, XU Lei, TAN Hai-gang. Algal Polysaccharides Inhibits Proliferation and Migration of Liver Cancer Cell Hep3B Via Down-regulation of EMP Pathway[J]. China Biotechnology, 2017, 37(9): 31-40.

Viewed

Full text

Abstract

Cited

Shared

Discussed