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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2019, Vol. 39 Issue (7): 56-64    DOI: 10.13523/j.cb.20190708
    
Enhanced Biomass Production and Lipid Accumulation by Co-cultivation of Chlorella vulgaris with Azotobacter Mesorhizobium sp.
Zhi-jin WEI,Xiao LI,Hao-nan WANG,Yong-hao YIN,Li-jun XI,Bao-sheng GE()
Center for Bioengineering and Biotechnology,China University of Petroleum (East China),Qingdao 266580,China
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Abstract  

Microalgal oil is an important functional oil, but also one of the major sources for bio-diesel production. In order to solve the contradiction of algae growth and lipid accumulation, the Chlorella vulgaris were co-cultured with azotobacter at different initial ratio under nitrogen source deficiency conditions, and the impact of consortium system growth, lipid accumulation based on the dry weight, lipid content, and fatty acids composition in the incubation period were evaluated. The results showed that the biomass and lipid content of algae were significantly higher than that of the pure culture of axenic C.vulgaris when C. vulgaris and B2.3 strains were inoculated at the ratio of 70∶1. The biomass, lipid content, lipid productivity, neutral lipid content and neutral lipid productivity of algae in the co-cultures were 1.68g/L, 45.2%, 75.94mg/(L·d), 23.0% and 38.65mg/(L·d), respectively. And the biomass concentration and lipid content in the co-culture system were 66.3% and 47.7% higher than that of the axenic pure algal cultures, respectively. Furthermore, the consortium system significantly increased the proportion of C18∶1 fatty acids. It was indicated that the co-cultivation of algae-bacteria system can effectively contribute to the quality and quantity of microalgal bio-oil, and has great potential for production of bio-diesel.

Key words： Co-culture      Chlorella vulgaris      Biomass      Lipid accumulation     
Received: 06 December 2018      Published: 05 August 2019
ZTFLH:  Q819  
Corresponding Authors: Bao-sheng GE     E-mail: gebaosheng@upc.edu.cn
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Zhi-jin WEI
Xiao LI
Hao-nan WANG
Yong-hao YIN
Li-jun XI
Bao-sheng GE
Cite this article:

Zhi-jin WEI,Xiao LI,Hao-nan WANG,Yong-hao YIN,Li-jun XI,Bao-sheng GE. Enhanced Biomass Production and Lipid Accumulation by Co-cultivation of Chlorella vulgaris with Azotobacter Mesorhizobium sp.. China Biotechnology, 2019, 39(7): 56-64.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190708     OR     https://manu60.magtech.com.cn/biotech/Y2019/V39/I7/56

Fig.1 The biomass and specific growth rate of C.vulgaris in co-culture with Mesorhizobium sp. after the 10d cultivation
Fig.2 The lipid content and productivity of C.vulgaris in co-culture with Mesorhizobium sp. after the 10d cultivation
Fig.3 The neutral lipid content and productivity of C.vulgaris in co-culture with Mesorhizobium sp.after the 10d cultivation
Fig.4 The fatty acid proportion of C.vulgaris in co-culture with Mesorhizobium sp. after the 10d cultivation
Fig.5 The protein concentration of in co-culture with Mesorhizobium sp. after the 10d cultivation
Fig.6 The polysaccharide concentration of C.vulgaris in co-culture with Mesorhizobium sp. after the 10d cultivation
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