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Abstract Liquid chip technology have been licensed to be used in clinic because of its advantage of high-throughput、high-sensitivity、good signal to noise ratio、reaction in liquid phase、convenient operation and short time consuming and so on. This study focused on the optimization of a liquid chip system for the detection of serum biomarkers of colorectal tumour and initial application in the detection of CEA. The optimized reaction conditions of liquid chip were determined through orthogonal design . The results showed that the reaction time of the coated antibody and the antigen,、the microspheres and the biotinylated detecion antibody and the complexes and avidin-PE was 1 hour, 1hour and 15minutes respectively.The optimized dilution of the biotinylated detection antibody was 1:300 and the optimized concentration of avidin-PE was 12μg/ml. Totally 55 clinical samples were detected by the liquid chip and by Enzyme-Linked Immunosorbent Assay (ELISA) simultaneously and the results of the two methods were compared. The results of the two methods showed good correlation in both positive and negative samples but the detection limits and the dynamic ranges of the liquid chip method were more sensitive and wider than those of the ELISA. The multiple tumour biomarkers may be detected simultaneously and the time of clinical test and manpower requirements were reduced by the liquid chip method．
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Received: 21 August 2006
Published: 25 December 2006
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