Separation and Purification of Coenzyme Q10 from microbial fermentation by High-speed Counter-Current Chromatography

China Biotechnology

技术与方法

Separation and Purification of Coenzyme Q10 from microbial fermentation by High-speed Counter-Current Chromatography

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Abstract High-speed counter-current chromatography (HSCCC) is applied for the first time in purification of Coenzyme Q10 (CoQ10) from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane-acetonitrile- dichloromethane (12:7:3.5, v/v/v) is selected by analytical HSCCC and used for preparative purification of CoQ10 from 500 mg of the crude extract. The separation yielded 130 mg of CoQ10 at high purity of over 98%. The overall results of the present studies show advantages of HSCCC in the purity, recovery and yield of CoQ10 over the silica gel chromatography with subsequent crystallization.

Key words： Coenzyme Q10 Separation and Purification High-speed countercurrent chromatography (HSCCC)

Received: 30 March 2006 Published: 25 August 2006

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Cite this article:

. Separation and Purification of Coenzyme Q10 from microbial fermentation by High-speed Counter-Current Chromatography. China Biotechnology, 2006, 26(08): 88-92.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I08/88

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