Abstract Objective To construct an expression system containing the N-terminal segment of Thrombospondin-1(TSP-1) in E.Coli.. To investigate the activity of TSF. Methods thbs1 gene fragment was amplified from human fetal cord vein endothelial cells and inserted into plasmid pET32c(+) which was then transfected and expressed in E.Coli.. Investigate the effect of recombinant TSF to the proliferation of ECV304 in vitro with MTT. The expression level of CD36 was assayed by FACS. Results Recombinant TSF was purified. TSF could restrain the proliferation of ECV304 with CD36 low-expression. Conclusions Low dose of rTSF was a latent assistant treatment of anti-cancer.
Received: 28 September 2005
Published: 25 April 2006
