Progress in the Synthesis of Artificial Antigen

doi:10.13523/j.cb.20181209

China Biotechnology

2018, Vol. 38

Issue (12): 65-75 DOI: 10.13523/j.cb.20181209

Orginal Article

Progress in the Synthesis of Artificial Antigen

LI Rong-qing,QUAN Chun-shan(

),ZHANG Li-ying(

),LIU Jia-lu,ZHANG Xiang,SHANG Fei

Key Laboratory of the State Ethnic Affairs Commission-Ministry of Education,School of Life Science,Dalian Nationalities University,Dalian 116600,China

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Abstract

The immunoassay with small molecular as a hapten has been used in many fields, such as food and drug, environmental protection and so on. It has obtained more ideal detection results. The small molecular must be coupled with the carrier to form an artificial antigen, which can indirectly induce the proliferation and differentiation of B cells by using the epitopes of T cells, and then produce specific antibodies. The synthesis of high efficient artificial antigen is the precondition and key to ensure immune analysis. Based on this, the design and synthesis methods, the selection of the carrier, the coupling method of the hapten and the carrier, the purification and identification methods of the artificial antigen, both here and abroad are reviewed.

Key words： Hapten Artificial antigen Selection of carrier Purification of antigen Identification of antigen

Received: 25 June 2018 Published: 10 January 2019

ZTFLH:

Q816

Corresponding Authors: Chun-shan QUAN,Li-ying ZHANG E-mail: mikyeken@dlnu.edu.cn;zhangly@dlnu.edu.cn

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	Rong-qing LI
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	Xiang ZHANG
	Fei SHANG

Cite this article:

LI Rong-qing,QUAN Chun-shan,ZHANG Li-ying,LIU Jia-lu,ZHANG Xiang,SHANG Fei. Progress in the Synthesis of Artificial Antigen. China Biotechnology, 2018, 38(12): 65-75.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181209 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I12/65

Fig.1 Phosphohistidine structural analogues used as hapten

Fig.2 Stilbestrol hapten

Fig.3 Effect of the linking spacer length on the coupling ratio of artificial antigen

Fig.4 Norepinephrine hapten(a) and 3-amino-2-oxazolidinone hapten (b)

Fig.5 Synthetic route of hapten TBBPA-D2


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