Bioengineering Application of Ferritin

doi:10.13523/j.cb.20180611

China Biotechnology

2018, Vol. 38

Issue (6): 77-85 DOI: 10.13523/j.cb.20180611

Bioengineering Application of Ferritin

Ling WANG,Yang WU,Sheng ZHANG,Hao QI(

)

Key Laboratory of Systems Bioengineering of Ministry of Education, SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China

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Abstract

Widely conserved iron-storage protein plays crucial role in ferric ion metabolism maintaining iron homeostasis, resisting oxidative stress and eliminating other toxic effects of excessive metal ions. With gaining the knowledge from structural and molecular studies, iron-storage protein complex in which iron cluster formed from absorbing free iron has been successfully applied in fields of biomedical engineering, nanomaterial, and biomolecule imaging. Herein, the recent progresses in studying the catalytic mechanism of iron cluster formation are briefly introduced. And the cutting-edge bioengineering applications in which iron-storage protein were engineered as a versatile molecular scaffold for presenting special chemicals, or natural magnetic particle for constructing remote control molecular machine are reviewed, although related researches has arisen academic argument.

Key words： Ferritin Magnetic particle Molecular scaffold

Received: 29 January 2018 Published: 06 July 2018

ZTFLH:

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Corresponding Authors: Hao QI E-mail: haoq@tju.edu.cn

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Cite this article:

Ling WANG,Yang WU,Sheng ZHANG,Hao QI. Bioengineering Application of Ferritin. China Biotechnology, 2018, 38(6): 77-85.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180611 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I6/77

Fig.1 The structure of ferritin family and ferritin subunit

Fig.2 The mechanism of ferritin ferroxidase center

Fig.3 The schematic diagram of artificial evolution of ferritin (a) Selection of ferritin mutant strains with yeast cells as the expression vector (b) Screening of ferritin mutant strains with E. coli expression vector

Fig.4 The methods of ferritin modification

Fig.5 Ferritin magnetic control switch (a) Radio waves, ferritin heat-driven switch (b) Magnetic fields, ferritin torque-actuated switch

Fig.6 The conditions of ferritin disassembly and assembly


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