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The substrate specificity of mutant cyclic imide hydrolases |
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Abstract Abstract: To explore the effect of C-terminal region residues on the substrate specificity of a novel cyclic imide hydrolase (CIH), a recombinant cyclic imide hydrolase (CIH293) and its mutant enzymes deleted or substituted at C-terminus (CIH291, CIH290, KK292-293EE) were prepared by gene cloning. Their substrate specificity and kinetic parameters were analyzed by both the spectrophotometric assay and high-performance liquid chromatography. Results show that the substrate specificity of mutant enzymes was not obviously changed, but slightly low for the substrate affinity, compared with the wild-type enzyme, CIH293.
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Received: 19 January 2007
Published: 25 June 2007
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