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The Regulation on Polyols Production by Trichosporonoides oedocephalis with HOG1 Inhibitors and Its Mechanism |
Li-na GU1,Liang-zhi LI1,2(),Wei-qiang GUO1,Jing-sheng GU1,Xue-mei YAO1,Xin JU1 |
1 School of Chemistry, Biology, and Materials Engineering, Suzhou University of Science and Technology,Suzhou 215009, China 2 Department of Biological Sciences, National University of Singapore, Singapore 117543 |
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Abstract Objective: To regulate the production of polyols by Trichosporonoides oedocephalis employing HOG1 inhibitors. Methods: SB239063, SB202190 and SB203580 were added into the medium to conduct fermentation experiments, and the influence of three inhibitors on the fermentation was compared. Results: The experimental results showed SB239063 would decrease the ctGPD enzyme activity of Trichosporonoides oedocephalis, and enhance erythritol reductase(ER)enzyme activity at the same time.Furthermore, the Western blot analysis of HOG1 and phospho-HOG1demonstrated that SB239063 also inhibited the dephosphorylation of HOG1. After 120 h fermentation, the addition of 10μmol/L SB239063 decreased glycerol production by 20.57% and increased erythritol production by 31.16%.In other words, the conversion rate of glucose was increased by 24.73%. Conclusion: SB239063 weakened the ability to produce glycerol of Trichosporonoides oedocephalis and therefore promoted erythritol yield.
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Received: 29 May 2017
Published: 16 December 2017
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