Heterologous Expression of <i>CiRS</i> Gene Eenhances the Antioxidant Capacity of <i>Arabidopsis</i> by Increasing the Content of Resveratrol

doi:10.13523/j.cb.20171206

China Biotechnology

2017, Vol. 37

Issue (12): 27-33 DOI: 10.13523/j.cb.20171206

Orginal Article

Heterologous Expression of CiRS Gene Eenhances the Antioxidant Capacity of Arabidopsis by Increasing the Content of Resveratrol

Fei-yun YANG^1,²,Yan-yan WU²,Shuang CUI¹,Xiu-juan ZHANG³,Rui-gang WANG¹,Guo-jing LI¹(

)

1 College of Life Sciences, Inner Mongolia Agricultural University, Hohhot　010018, China
2 College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot　010018, China

3 Inner Mongolia Autonomous Region Biotechnology Research Institute, Hohhot　010070, China

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Abstract

Resveratrol is a member of stilbenoids with disease resistant activity for plants and exhibits a wide range of important biological and pharmacological properties for human which has received extensive attention in the fields of agriculture, medicine, foods, cosmetics and so on. Resveratrol synthase (RS) is an exclusive necessary enzyme in the pathway of resveratrol biosynthesis which determines the synthesis of resveratrol in plants. CiRS, a RS gene isolated from Caragana intermedia, was transferred into Arabidopsis. Total flavonoids experimental results showed that the total flavonoids content of wild type was significantly higher than that of the transgenic lines. HPLC method was used to analyze the resveratrol content in transgenic plants which maximum content was 335μg/g FW. Accumulation of malondialdehyde (MDA) after UV treatment in transgenic plants was significantly less than the wild type. DPPH free radical scavenging ability of transgenic plants extraction was higher than the wild type. Taken together, these results indicated that the antioxidant activity of transgenic plants was enhanced with the expression of CiRS gene which synthesise resveratrol with flavonoid substrates.

Key words： Resveratrol synthase Caragana intermedia Resveratrol Transgenic Arabidopsis

Received: 24 August 2017 Published: 16 December 2017

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	Fei-yun YANG
	Yan-yan WU
	Shuang CUI
	Xiu-juan ZHANG
	Rui-gang WANG
	Guo-jing LI

Cite this article:

Fei-yun YANG,Yan-yan WU,Shuang CUI,Xiu-juan ZHANG,Rui-gang WANG,Guo-jing LI. Heterologous Expression of CiRS Gene Eenhances the Antioxidant Capacity of Arabidopsis by Increasing the Content of Resveratrol. China Biotechnology, 2017, 37(12): 27-33.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20171206 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I12/27

Fig.1 The pCanG-CiRS recombinant vector

Fig.2 Expression analysis of CiRS in transgenic Arabidopsis

Fig. 3 The content of total flavonoids in different Arabidopsis lines
(a) The standard curve of rutin (b) The content of total flavonoids in different Arabidopsis lines ^** indicates significant difference among samples at 0.01 level

Fig.4 The resveratrol chromatogram map of different Arabidopsis lines
(a) Standard sample (b) The resveratrol chromatogram map of different Arabidopsis lines black line: WT; green line: OE-1; red line: OE-16; blue line: OE-17

Table 1 The resveratrolcontent of different Arabidopsis lines

Fig.5 The content of MDA in different Arabidopsis lines
^* Indicates significant difference among samples at 0.05 level; ^** Indicates significant difference among samples at 0.01 level

Fig. 6 The scavenging rate of DPPH radical of different Arabidopsis lines
(a) The standard curve of DPPH (b) The scavenging rate of DPPH radical of different Arabidopsis lines ^** Indicates significant difference among samples at 0.01 level

Fig.7 Trolox standard curve for scavenging rate of DPPH radical


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