中国生物工程杂志

The yeast one hybrid system is the new type system deriving from the yeast two hybrid system to study the interaction between DNA and protein. This review briefly introduced the principle and the technical line of this system, and particularly summarized the progress of this system in plant osmotic stress-resist gene engineering field，such as cloning the transcription factor genes of osmosis resistance type; confirming the interaction between known DNA and protein; making sure the localization of the confirmed DNA binding domain with the interaction; also validating the transcription activation. Furthermore, we analyzed the problems existing in this system on the research of plant osmotic stress-resist gene engineering, as well as forecasting the development prospect according to our research.