|
|
|
| Research on Stress-inducible Expression Characteristics of Sorbitol-6- phosphate Dehydrogenase Promoter from Apple |
| XIA Hui1,2, LIU Lei3, WANG Xiu1, SHEN Yan-qiu1, GUO Yu-lun1, LIANG Dong1,2 |
1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China;
3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China |
|
|
|
Abstract In order to understand stress-inducible expression characteristics of sorbitol-6-phosphate dehydrogenase (S6PDH) promoter (S6PDHp) from apple, a series of 5' deletions of S6PDH promoter were fused with GUS reporter gene by Gateway and introduced into arabidopsis byagrobacterium-mediated transformation. The expression characteristics of S6PDHp were validated by GUS activity changed in transgenetic Arabidopsis against low temperature and exogenous ABA. Molecular detection analysis revealed that four 5' deletions of S6PDHp were fused with GUS reporter gene successfully (i.e. pGWB433-S6PDHp1, pGWB433-S6PDHp2, pGWB433-S6PDHp3 and pGWB433-S6PDHp4) and relevant transgenetic Arabidopsis were obtained. In low temperature treatment, GUS activity in pGWB433-S6PDHp3 transgenetic line increased markedly, while there were obvious change in other deletion transgenetic lines. In exogenous ABA treatment, GUS activity in others deletions transgenetic lines increased dramatically except pGWB433-S6PDHp4. These results suggest that S6PDHp can be induced by low temperature or exogenous ABA, however, there were different response in every deletions. These indicated that positive and negative cis-regulatory elements in response to abiotic stress may co-existed in the promoter region (-2 396bp to -236bp) of S6PDH.
|
|
Received: 21 March 2017
Published: 25 June 2017
|
|
|
|
|
[1] Loescher W H. Physiology and metabolism of sugar alcohols in higher plant. Physiology Plantarum, 1987, 70(3):553-557.
[2] Zhou R, Cheng L L, Wayne R. Purification and characterization of sorbitol-6- phosphate phosphatase from apple leaves. Plant Science, 2003, 165(1):227-232.
[3] Lo Biancoa R, Riegera M, Sung S S. Effect of drought on sorbitol and sucrose metabolism in sinks and sources of peach. Physiology Plantarum, 2000, 108(1):71-78.
[4] Cui S M, Sadayoshi K, Ogawa Y, et al. Effects of water stress on sorbitol content in leaves and roots, anatomical changes in cell nuclei, and starch accumulation in leaves of young peach trees. Journal of Japanese Society Horticulture Science, 2004, 73(1):25-30.
[5] Suleman P, Steiner P W. Relationship between sorbitol and solute potential in apple shoots relative to fire blight symptom development after infection by Erwinia amylovora. Phytopathology, 1994, 84(10):1244-1250.
[6] Kanayama Y, Watanabe M, Moriguchi R, et al. Effects of low temperature and abscisic acid on the expression of the sorbitol-6- phosphate dehydrogenase gene in apple leaves. Journal of Japanese Society Horticulture Science, 2006, 75(1):20-25.
[7] Tao G, Suzuki Y, Uratsu S L, et al. Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(49):18842-18847.
[8] Cheng L L, Zhou R, Reidel E J, et al.. Antisense inhibition of sorbitol synthesis leads to up-regulation of starch synthesis without altering CO2 assimilation in apple leaves. Planta, 2005, 220(5):767-776.
[9] Kanayama Y, Mori H, Imaseki H, et al. Nucleotide sequence of a cDNA encoding sorbitol-6-phosphate dehydrogenase from apple. Plant Physiology, 1992, 100(3):1607-1608.
[10] 梁东, 马锋旺, 张军科, 等.苹果6-磷酸山梨醇脱氢酶基因cDNA克隆及其植物表达载体构建. 农业生物技术学报, 2006, 14(4):635-636. Liang D, Ma F W, Zhang J K, et al. Cloning of sorbitol-6-phosphate dehydrogenase S6PDH cDNA from apple and construction of its plant expression vector. Journal of Agricultural Biotechnology, 2006, 14(4):635-636.
[11] Meng Y L, Xu X F, Khanizadeh S, et al. The contribution of abscisic acid to sorbitol accumulation in drought-stressed Malus hupehensis. Journal of Food, Agriculture & Environment, 2008, 6(2):319-326.
[12] Qi X T, Zhang, Y X, Chai T Y. Characterization of a novel plant promoter specifically induced by heavy metal and identification of the promoter regions conferring heavy metal responsiveness. Plant Physiology, 2007, 143(1):50-59.
[13] Xu W R, Yu Y H, Ding J H, et al. Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata. Planta, 2010, 231:475-487.
[14] Li J, Li M J, Liang D, et al. Expression patterns and promoter characteristics of the gene encoding Actinidia deliciosa L-galactose-1-phosphate phosphatase involved in the response to light and abiotic stresses. Molecular Biology Reporter, 2013, 40(2):1473-1485.
[15] Liang D, Cui M, Wu S, et al. Genomic structure, sub-cellular localization, and promoter analysis of the gene encoding sorbitol-6-phosphate dehydrogenase from apple. Plant Molecular Biology Reporter, 2012, 30(4):904-914.
[16] Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions:beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. European Molecular Biology Organization Jouranl, 1987, 6(13):3901-3907.
[17] White A J, Dunn M A, Brown K, et al. Comparative analysis of genomic sequence and expression of a lipid transfer protein gene family in winter barley. Journal of Experimental Botany, 1994, 45(12):1885-1892.
[18] Yamaguchi S K, Shinozaki K. Arabidopsis DNA encoding two desiccation~responsive rd29 genes. Plant Physiology, 1993, 101(3):1119-1120.
[19] Li J, Liang D, Li M J, et al. Light and abiotic stresses regulate the expression of GDP-l galactose phosphorylase and levels of ascorbic acid in two kiwifruit genotypes via light responsive and stress inducible cis-elements in their promoters. Planta, 2013, 238(3):535-547. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
