|
|
|
| Effect of CX3CR1 on Human Hepatocellular Carcinoma Cells and Its Mechanism |
| FAN Meng-tian, CHEN Si-cheng, GUO Yang-liu, LI Ya, SUN Yan-ting, LI Wang, SHI Qiong |
| Key laboratory of Clinical Laboratory Diagnostics, Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China |
|
|
|
Abstract Aim:To investigate the effect of CX3CR1 on the proliferation migration and invasion abilities of hepatocellular carcinoma(HCC) cell lines and its mechanism. Methods:The expression of CX3CR1 at mRNA and protein levels of 7721,HepG2 and human normal liver epithelial cells LO2 was detected by Q-PCR and Western blot. The 7721 cells were transfected with the overexpression plasmid of CX3CR1;the HepG2 cells were transfected with siRNA of CX3CR1,and the expression of CX3CR1 at mRNA and protein levels were detected by Q-PCR and Western blot. The cell proliferation ability was detected by MTT and clone formation assay. The migration and invasion ability were detected by Wound-healing and Transwell assays. The protein levels of PI3K/AKT and MAPK/ERK were detected by Western blot. Results:The expression of CX3CR1 was lower in 7721 cells than that in HepG2 cells. After overexpression plasmid was transfected into 7721 cells,the expression of CX3CR1 was up-regulated; cell proliferation migration and invasion abilities were increased. Meanwhile, the levels of p-ERK and p-AKT were up-regulated. After siRNA was thansfected into HepG2 cells,the expression of CX3CR1 was down-regulated,and cell proliferation, migration and invasion abilities were decreased, with the levels of p-ERK and p-AKT down-regulated. Conclusion:CX3CR1 can promote the proliferation, migration and invasion abilities of hepatocellular carcinoma(HCC) cells. The activation of PI3K/AKT and MEK/ERK signaling pathway may play an important role in these processes.
|
|
Received: 13 December 2016
Published: 25 June 2017
|
|
|
|
|
[1] Moriguchi M, Umemura A.Current status and future prospects of chemotherapy for advanced hepatocellular carcinoma. Clin J Gastroenterol, 2016,9(4):184-190.
[2] Kufareva I.Chemokines and their receptors:insights from molecular modeling and crystallography. Curr Opin Pharmacol, 2016, 30:27-37.
[3] Huang F,Geng X P.Chemokines and hepatocellular carcinoma.World Journal of Gastroenterology,2010, 16(15):1832-1836.
[4] Ren H, Zhao T, Sun J.The CX3CL1/CX3CR1 reprograms glucose metabolism through HIF-1 pathway in pancreatic adenocarcinoma. J Cell Biochem,2013, 114(11):2603-2611.
[5] Yao X, Qi L, Chen X.Expression of CX3CR1 associates with cellular migration, metastasis, and prognosis in human clear cell renal cell carcinoma.Urologic Oncology,2013, 32(2):162-170.
[6] Lv C Y, Zhou T, Chen W.Preliminary study correlating CX3CL1/CX3CR1 expression with gastric carcinoma and gastric carcinomaperineural invasion. World Journal of Gastroenterology, 2014, 20(15):4428-4432.
[7] Liu J F, Tsao Y T, Hou C H.Fractalkine/CX3CL1 induced intercellular adhesion molecule-1-dependent tumor metastasis through the CX3CR1/PI3K/Akt/NF-κB pathway in human osteosarcoma. Oncotarget, 2016, Aug 12.
[8] Shao Y, Guan J Z. Advances in the treatment of primary liver.Infectious Disease Information, 2016,29(4):248-252.
[9] Brunot A, Le Sourd S. Hepatocellular carcinoma in elderly patients:challenges and solutions.J Hepatocell Carcinoma,2016, 3:9-18.
[10] Shah A D, Bouchard M J.Interstitial fluid flow increases hepatocellular carcinoma cell invasion through CXCR4/CXCL12 and MEK/ERK signaling. PLoS One,2015, 10(11):e0142337.
[11] Du D, Liu Y. The effects of the CCR6/CCL20 biological axis on the invasion and metastasis of hepatocellular carcinoma. Int J Mol Sci, 2014,15(4):6441-6452.
[12] Sadeghi M, Lahdou I.Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma. Br J Cancer, 2015,113(5):756-762.
[13] Yeung O W, Lo C M. Alternatively activated (M2) macrophages promote tumour growth and invasiveness in hepatocellular carcinoma. J Hepatol, 2015, 62(3):607-616.
[14] Jamieson W L. CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow:potential role in prostate cancer bone tropism. Cancer Res, 2008, 68(6):1715-1722.
[15] Luo Wen, Lin Yuanlong. miRNA-296-3p modulates chemosensitivity of lung cancer cells by targeting CX3CR1. Am J Transl Res, 2016, 8(4):1848-1851. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
