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| Dissolved Carbon Dioxide Effects on Glucoamylase Synthesis of Aspergillus niger in Batch and Chemostat Cultures |
| WANG Li-qun, LU Hong-zhong, CHU Ju, WANG Yong-hong |
| State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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Abstract The effect of different levels of elevated dissolved CO2 availability(CO2 stress) was investigated in batch and chemostat cultures of Aspergillus niger by gassing with CO2-enriched air. Under batch cultures, higher elevated dissolved CO2 availability in culture medium resulted in a more significant decrease in specific growth rate, whereas it appeared to have a positive effect on glucoamylase synthesis. Under chemostat cultures with low dilution rate (D1=0.05/h), high levels of dissolved CO2 led to no reduction of specific growth rate, but an increase of glucoamylase production. Whereas at high dilution rate (D2=0.08/h), both substrate uptake and cell grow were more severely inhibited by more elevated dissolved CO2. The effect of elevated dissolved CO2 availability on the fermentation process depends on CO2 levels as well as dilution rate. Therefore, the influence of carbon dioxide on cell growth and glucoamylase production dependent on CO2 levels, cultivation mode and specific metabolic activity, which would be helpful for the design of specific growth rate in scale-up of glucoamylase fermentation by Aspergillus niger.
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Received: 25 October 2016
Published: 25 January 2017
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