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中国生物工程杂志

China Biotechnology
China Biotechnology  2007, Vol. 27 Issue (1): 28-34    DOI:
    
Construction of SSR genetic Map and its QTL analysis of agronomic traits with sequenced varieties
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Abstract  A F2 population, included 90 lines, which derived from the cross between the completely sequenced japonica variety Nipponbare and the indica variety GuangLuAi-4, was applied to construct a genetic linkage framework map. The map covered 1737.81cM,of total genomic length, and included 148 SSR loci; an average distance of 11.90 cM。The phenotypic values of 6 agronomic traits including number of tillers (Tn), number of panicle (Pn), number of effective tillers (Etn), main panicle length (Mpl), plant height (Ph), and flag length (Fl) were surveyed with three replication (Table 1 and Fig.1). Correlation coefficients between these traits were calculated by means of the software of Excel 2000 were found to be similar to other research conclusions (Table 2). was applied to detect QTLs with Mapmaker/QTL1.1b. Under the condition that LOD>2.2 and P<0.005, 29 QTLs were detected, which distributed on all rice chromosomes. The phenotypic variations (V.E.) explained by individual QTL were ranged from 11.1% to 42.9%, among which 11 QTLs explained V.E. by more than 20%(Table 3 and Fig.2). The molecular basis of inheritance and the applications of QTL for rice agronomic traits with sequenced rice materials as mapping parents were discussed.

Received: 27 November 2006      Published: 10 May 2010
Cite this article:

. Construction of SSR genetic Map and its QTL analysis of agronomic traits with sequenced varieties. China Biotechnology, 2007, 27(1): 28-34.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2007/V27/I1/28

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