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| The Streptoverticillium mobaraense Mutagenesis Using Atmospheric Pressure Plasma at Room Temperature (ARTP) Method |
| TIAN Shu-cui1, NIU Yan-ning1, CHANG Zhong-yi1, GAO Hong-liang1, BU Guo-jian2, JIN Ming-fei1 |
1 Life Science College of East China Normal University, Shanghai 200241, China;
2 Taixing Dongsheng Food Science and Technology Limited Company, Taixing 225411, China |
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Abstract To get microorganism transglutaminase (MTG) high yield strains, the spores of Streptoverticillium mobaraense strain HS47 were mutagenized using atmospheric pressure plasma at room temperature (ARTP) method.The fatality rate strengthand positive mutation rate result of the strains reveal that when the power is 120W, handling distance is 2mm, working flow is 10slpm,the optimum time for the plasma heliumto mutagenized spores of S.mobaraense HS47 was 30s.Spread the mutagenic spores diluent to tablets and screen single colonies using 96-well plates high-throughput screening method. Pick out the high yield mutant strains to go on with two rounds of test tube screening. Among the process, keep the separation and purification of strains. Finally, one high yield strain M-8 was found. Its MTG enzyme activity was 5.1U/ml,increased by 82% compared to the starting strain HS47 whose MTG enzyme activity was only 2.8U/ml.The reaults of shaking flask fermentation of strain M-8 show that its increase in the enzyme activity results from the increase of MTG secretion of unit strain.The MTG enzyme activity of M-8 of eight batches have no significant difference, so the strain has good genetic stability. The found of M-8 provides strain support and theoretical support for industrialized production of MTG.
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Received: 14 March 2016
Published: 25 September 2016
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| [1] Santos M, Torné J M. Recent patents on transglutaminase production and applications:a brief review. Recent Patents on Biotechnology, 2009, 3(3):166-174.
[2] Ando H, Adachi M, Umeda K, et al. Purification and characteristics of a novel transglutaminase derived from microorganisms. Agricultural & Biological Chemistry, 1989, 53(10):2613-2617.
[3] 张莹, 郭琛琛, 黑东燕, 等. 复合诱变法选育谷氨酰胺转胺酶高产菌株. 食品科学, 2014, 35(11):139-142. Zhang Y, Guo C H CH, He D Y, et al.Screening and characterization of high-yield microbial transglutaminase-producing mutants induced by a novel method.Food Science,2014, 35(11):139-142.
[4] 郭玮婷, 张慧, 查东风, 等. 产耐高温谷氨酰胺转胺酶菌株的快速筛选方法. 中国生物工程杂志, 2015, 35(8):83-89. Guo W T, Zhang H, Zha D F,et al.A rapid method of screening for thermostabletransglutaminase from Streptomyces mobaraensis.China Biotechnology, 2015, 35(8):83-89.
[5] Zhang X, Zhang X F, Li H P, et al. Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Applied Microbiology & Biotechnology, 2014, 98(12):5387-5396.
[6] Lu Y, Wang L, Ma K, et al. Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP). Biochem Eng J, 2011, 55(1):17-22.
[7] 金丽华, 方明月, 张翀, 等. 常压室温等离子体快速诱变产油酵母的条件及其突变株的特性. 生物工程学报, 2011, 27(3):461-467. Jin L H, Fang M Y, Zhang C H,et al.Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants. Chinese Journal of Biotechnology, 2011, 27(3):461-467.
[8] 刘雅清, 侯孝仑, 郭玮婷, 等. 甲壳素促进茂源链霉菌发酵产酶. 中国生物工程杂志, 2015,35(6):40-45. Liu Y Q, Hou X L,guo W T,et al.Enhancement of transglutaminase production in Streptoverticillium mobaraensis as achieved by treatment with chitin.China Biotechnology,2015,35(6):40-45.
[9] Grossowicz N, Wainfan E, Borek E, et al. The enzymatic formation of hydroxamic acids from glutamine. J Biol Chem, 1950, 187(1):111-125.
[10] 陈佳, 金明飞, 谭玉静, 等. NaCl对轮枝链霉菌产谷氨酰胺转胺酶的促进作用研究. 中国生物工程杂志, 2013, 33(2):54-58. Chen J, Jin M F,Tan Y J,et al.Enhancement of transglutaminase production in Streptoverticillium mobaraensis as achieved by treatment with NaCl.China Biotechnology,2013, 33(2):54-58.
[11] Li G, Li H P, Wang L Y, et al. Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium. Appl Phys Lett, 2008, 92(22):1504-1507.
[12] Hui-Lin Y, Li P, Ying L. Purification and on-column activation of a recombinant histidine-tagged pro-transglutaminase after soluble expression in Escherichia coli. Bioscience Biotechnology & Biochemistry, 2009, 73(11):2531-2534.
[13] Wang Q, Feng L R, Wei L, et al. Mutation breeding of lycopene-producing strain blakeslea trispora by a novel atmospheric and room temperature plasma (ARTP). Applied Biochemistry & Biotechnology, 2014, 174(1):452-460. |
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